Structure of PDB 5btc Chain C

Receptor sequence

>5btcC (length=487) Species: 83332 (Mycobacterium tuberculosis H37Rv)

IEPVDIEQEMQRSYIDYAMSVIVGRALPEVRDGLKPVHRRVLYAMFDSGF
RPDRSHAKSARSVAETMGNYHPHGDSSIYDSLVRMAQPWSLRYPLVDGQG
NFGSPGNDPPAAMRYTEARLTPLAMEMLREIDEETVDFIPNYDGRVQEPT
VLPSRFPNLLANGSGGIAVGMATNIPPHNLRELADAVFWALENHDADEEE
TLAAVMGRVKGPDFPTAGLIVGSQGTADAYKTGRGSIRMRGVVEVEEDSR
GRTSLVITELPYQVNHDNFITSIAEQVRDGKLAGISNIEDQSSDRVGLRI
VIEIKRDAVAKVVINNLYKHTQLQTSFGANMLAIVDGVPRTLRLDQLIRY
YVDHQLDVIVRRTTYRLRKANERAHILRGLVKALDALDEVIALIRASETV
DIARAGLIELLDIDEIQAQAILDMQLRRLAALERQRIIDDLAKIEAEIAD
LEDILAKPERQRGIVRDELAEIVDRHGDDRRTRIIAI

3D structure

• PDB: 5btc Crystal structure and stability of gyrase-fluoroquinolone cleaved complexes from Mycobacterium tuberculosis.
• Chain: C
• Resolution: 2.55 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	Y28 R128 Y129 I181 A182 G184 M185	Y14 R114 Y115 I167 A168 G170 M171
BS02	dna	C	R39 V51 H52 H85 H87 S95 R98 I181	R25 V37 H38 H71 H73 S81 R84 I167
BS03	dna	C	Y28 R128 Y129 I181 A182 G184 M185	Y14 R114 Y115 I167 A168 G170 M171
BS04	dna	C	R39 K49 H52 H85 H87 S95 R98 I181	R25 K35 H38 H71 H73 S81 R84 I167

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006265 DNA topological change

External links

• PDB: RCSB:5btc, PDBe:5btc, PDBj:5btc
• PDBsum: 5btc
• PubMed: 26792525
• UniProt: P9WG47|GYRA_MYCTU DNA gyrase subunit A (Gene Name=gyrA)