Structure of PDB 4zi6 Chain C

Receptor sequence

>4zi6C (length=481) Species: 85962 (Helicobacter pylori 26695)

MLKIKLEKTTFENAKAECSLVFIINKDFSHAWVKNKELLETFKYEGEGVF
LDQENKILYAGVKEDDVHLLRESACLAVRTLKKLAFKSVKVGVYTNALLE
NLKALFLGLKLGLYEYDTFKSNKKESVLKEAIVALELHKSLEKSAKEALK
YAEIMTESLNIVKDLVNTPPMIGTPVYMAEVAQKVAKENHLEIHVHDEKF
LEEKKMNAFLAVNKASLSVNPPRLIHLVYKPKKAKKKIALVGKGLTYDCG
GLSLKPADYMVTMKADKGGGSAVIGLLNALAKLGVEAEVHGIIGATENMI
GPAAYKPDDILISKEGKSIEVRNTDAEGRLVLADCLSYAQDLNPDVIVDF
ATLTGACVVGLGEFTSAIMGHNEELKNLFETSGLESGELLAKLPFNRHLK
KLIESKIADVCNISSSRYGGAITAGLFLNEFIRDEFKDKWLHIDIAGPAY
VEKEWDVNSFGASGAGVRACTAFVEELLKKA

3D structure

• PDB: 4zi6 Structural basis for substrate specificity of Helicobacter pylori M17 aminopeptidase.
• Chain: C
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K270 R344
• Catalytic site (residue number reindexed from 1): K255 R329
• Enzyme Commision number: 3.4.11.1: leucyl aminopeptidase.
  3.4.11.10: bacterial leucyl aminopeptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	C	K258 D263 D281 E342	K243 D248 D266 E327
BS02	ZN	C	D263 D340 E342	D248 D325 E327
BS03	BCT	C	A341 E342 G343 R344 L368	A326 E327 G328 R329 L353
BS04	NA	C	A461 G462 Y465	A446 G447 Y450

Gene Ontology

Molecular Function

• GO:0004177 aminopeptidase activity
• GO:0008235 metalloexopeptidase activity
• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019538 protein metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4zi6, PDBe:4zi6, PDBj:4zi6
• PDBsum: 4zi6
• PubMed: 26616008
• UniProt: O25294|AMPA_HELPY Cytosol aminopeptidase (Gene Name=pepA)