Structure of PDB 4wya Chain C

Receptor sequence

>4wyaC (length=419) Species: 83332 (Mycobacterium tuberculosis H37Rv)

GLTPEQIIAVDGAHLWHPYSSIGRSPVVAVAAHGAWLTLIRDGQPIEVLD
AMSSWWTAIHGHGHPALDQALTTQLRVMNHVMFGGLTHEPAARLAKLLVD
ITPAGLDTVFFSDSGSVSVEVAAKMALQYWRGRGLPGKRRLMTWRGGYHG
DTFLAMSICDPHGTDVLAAQVFAPQVPRDYDPAYSAAFEAQLAQHAGELA
AVVVEPVVQGAGGMRFHDPRYLHDLRDICRRYEVLLIFDEIATGFGRTGA
LFAADHAGVSPDIMCVGKALTGGYLSLAATLCTADVAHTISAGAAGALMH
GPTFMANPLACAVSVASVELLLGQDWRTRITELAAGLTAGLDTARALPAV
TDVRVCGAIGVIECDRPVDLAVATPAALDRGVWLRPFRNLVYAMPPYICT
PAEITQITSAMVEVARLVG

3D structure

• PDB: 4wya Fragment-Based Exploration of Binding Site Flexibility in Mycobacterium tuberculosis BioA.
• Chain: C
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y25 Y157 E220 D254 A257 K283 Y407
• Catalytic site (residue number reindexed from 1): Y19 Y148 E205 D239 A242 K268 Y392
• Enzyme Commision number: 2.6.1.62: adenosylmethionine--8-amino-7-oxononanoate transaminase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PLP	C	S123 G124 S125 Y157 H158 D254 I256 K283	S114 G115 S116 Y148 H149 D239 I241 K268
BS02	3VQ	C	P24 Y25 W64	P18 Y19 W55
BS03	PLP	C	P317 T318	P302 T303

Gene Ontology

Molecular Function

• GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
• GO:0008483 transaminase activity
• GO:0030170 pyridoxal phosphate binding

Biological Process

• GO:0009102 biotin biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4wya, PDBe:4wya, PDBj:4wya
• PDBsum: 4wya
• PubMed: 26068403
• UniProt: P9WQ81|BIOA_MYCTU Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (Gene Name=bioA)