Structure of PDB 4ras Chain C

Receptor sequence

>4rasC (length=692) Species: 391937 (Nitratireductor pacificus pht-3B)

MRLYSNRDRPNHLGPLALERLARVDDVVAQPARQPEDGFAASEDSLLGDV
EEYARLFTRFLDGPVAPLGDAIPDDPARRAENLKASAYFLDASMVGICRL
DPDDCDPSHTHALVFAVQFGREPEAGEAGAEWIRGTNAARTDMRCAEIAA
ILSGYVRWMGFPARGHFSGDAQVDLARLAVRAGLARVVDGVLVAPFLRRG
FRLGVVTTGYALAADRPLAPEGDLGETAPEVMLGIDGTRPGWEDAEEEKR
PLHMGRYPMETIRRVDEPTTLVVRQEIQRVAKRGDFFKRAEAGDLGEKAK
QEKKRFPMKHPLALGMQPLIQNMVPLQGTREKLAPTGKGGDLSDPGRNAE
AIKALGYYLGADFVGICRAEPWMYYASDEVEGKPIEAYHDYAVVMLIDQG
YETMEGASGDDWISASQSMRAYMRGAEIAGVMAAHCRRMGYSARSHSNAH
SEVIHNPAILMAGLGEVSRIGDTLLNPFIGPRSKSIVFTTDLPMSVDRPI
DFGLQDFCNQCRKCARECPCNAISFGDKVMFNGYEIWKADVEKCTKYRVT
QMKGSACGRCMKMCPWNREDTVEGRRLAELSIKVPEARAAIIAMDDALQN
GKRNLIKRWWFDLEVIDGVAGAPRMGTNERDLSPDRGDKIGANQKLAMYP
PRLQPPPGTTLDAVLPVDRSGGLAEYAAAETPAAARARLKSS

3D structure

• PDB: 4ras Reductive dehalogenase structure suggests a mechanism for B12-dependent dehalogenation.
• Chain: C
• Resolution: 2.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	C	R473 I474 L479 F511 C512 C515 C518 C568 P569	R469 I470 L475 F507 C508 C511 C514 C564 P565
BS02	SF4	C	C522 P523 I527 C548 C561 C564	C518 P519 I523 C544 C557 C560
BS03	B12	C	D289 F290 F291 Y379 Y426 I458 H459 N460 P461 I463 I474 D476 T477 L478 K488 S489 F535 Y538 K542 V545 T549 R552	D285 F286 F287 Y375 Y422 I454 H455 N456 P457 I459 I470 D472 T473 L474 K484 S485 F531 Y534 K538 V541 T545 R548

Gene Ontology

Molecular Function

• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

External links

• PDB: RCSB:4ras, PDBe:4ras, PDBj:4ras
• PDBsum: 4ras
• PubMed: 25327251
• UniProt: K2MB66