Structure of PDB 4oog Chain C

Receptor sequence

>4oogC (length=261) Species: 559292 (Saccharomyces cerevisiae S288C)

KWPPKLPEIQDLAIRARVFIHKSTIKDKVYLSGSEMINAHNERLEFLGDS
ILNSVMTLIIYNKFPDYSEGQLSTLRMNLVSNEQIKQWSIMYNFHEKLKT
NFDLKDENSNFQNGKLKLYADVFEAYIGGLMEDDPRNNLPKIRKWLRKLA
KPVIEEATRNQVALEKTDKLDMNAKRQLYSLIGYASLRLHYVTVKKPTAV
DPNSIVECRVGDGTVLGTGVGRNIKIAGIRAAENALRDKKMLDFYAKQRA
AIPRSESVLKD

3D structure

• PDB: 4oog Structure of a Eukaryotic RNase III Postcleavage Complex Reveals a Double-Ruler Mechanism for Substrate Selection.
• Chain: C
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 3.1.26.3: ribonuclease III.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	D245 E265 G266 S269 M273 S277 N278 K371 R372 Y375 S376 Y380 A381 K392 P393 R418 N419 I420 K421 R445 R450 S453 V454 L455 K456	D49 E69 G70 S73 M77 S81 N82 K175 R176 Y179 S180 Y184 A185 K196 P197 R222 N223 I224 K225 R249 R254 S257 V258 L259 K260
BS02	MG	C	D245 E320	D49 E124
BS03	MG	C	E241 E320	E45 E124
BS04	MG	C	E238 E241	E42 E45

Gene Ontology

Molecular Function

• GO:0003725 double-stranded RNA binding
• GO:0004525 ribonuclease III activity

Biological Process

• GO:0006364 rRNA processing
• GO:0006396 RNA processing

External links

• PDB: RCSB:4oog, PDBe:4oog, PDBj:4oog
• PDBsum: 4oog
• PubMed: 24703949
• UniProt: Q02555|RNT1_YEAST Ribonuclease 3 (Gene Name=RNT1)