Structure of PDB 4oiq Chain C

Receptor sequence

>4oiqC (length=1112) Species: 300852 (Thermus thermophilus HB8)

MEIKRFGRIREVIPLPPLTEIQVESYRRALQADVPPEKRENVGIQAAFRE
TFPIEEGLVLDFLEYRLGEPPFPQDECREKDLTYQAPLYARLQLIHKDTG
LIKEDEVFLGHIPLMTEDGSFIINGADRVIVSQIHRSPGVYFTPDPARPG
RYIASIIPLPKRGPWIDLEVEPNGVVSMKVNKRKFPLVLLLRVLGYDQET
LARELGAYGELVQGLMDESVFAMRPEEALIRLFTLLRPGDPPKRDKAVAY
VYGLIADPRRYDLGEAGRYKAEEKLGIRLSGRTLARFEDGEFKDEVFLPT
LRYLFALTAGVPGHEVDDIDHLGNRRIRTVGELMTDQFRVGLARLARGVR
ERMLMGSEDSLTPAKLVNSRPLEAAIREFFSRSQLSQFKDETNPLSSLRH
KRRISALGPGGLTRERAGFDVRDVHRTHYGRICPVETPEGANIGLITSLA
AYARVDELGFIRTPYRRVVGGVVTDEVVYMTATEEDRYTIAQANTPLEGN
RIAAERVVARRKGEPVIVSPEEVEFMDVSPKQVFSVNTNLIPFLEHDDAN
RALMGSNMQTQAVPLIRAQAPVVMTGLEERVVRDSLAALYAEEDGEVAKV
DGNRIVVRYEDGRLVEYPLRRFYRSNQGTALDQRPRVVVGQRVRKGDLLA
DGPASENGFLALGQNVLVAIMPFDGYNFEDAIVISEELLKRDFYTSIHIE
RYEIEARDTKLGPERITRDIPHLSEAALRDLDEEGVVRIGAEVKPGDILV
GRTSFKGESEPTPEERLLRSIFGEKARDVKDTSLRVPPGEGGIVVRTVRL
RRGDPGVELKPGVREVVRVYVAQKRKLQVGDKLANRHGNKGVVAKILPVE
DMPHLPDGTPVDVILNPLGVPSRMNLGQILETHLGLAGYFLGQRYISPIF
DGAKEPEIKELLAQAFEVYFGKRKGEGFGVDKREVEVLRRAEKLGLVTPG
KTPEEQLKELFLQGKVVLYDGRTGEPIEGPIVVGQMFIMKLYHMVEDKMH
ARSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQEMLTLK
SDDIEGRNAAYEAIIKGEDVPEPSVPESFRVLVKELQALALDVQTLDEKD
NPVDIFEGLASK

3D structure

• PDB: 4oiq GE23077 binds to the RNA polymerase 'i' and 'i+1' sites and prevents the binding of initiating nucleotides.
• Chain: C
• Resolution: 3.624 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	G1023 K1024 R1031 M1035	G1017 K1018 R1025 M1029
BS02	dna	C	R142 K167 W171 R243 R266 D326 L418 R422	R136 K161 W165 R237 R260 D320 L412 R416
BS03	peptide	C	E445 G446 R557 M560 N563 Q567 K846	E439 G440 R551 M554 N557 Q561 K840

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:4oiq, PDBe:4oiq, PDBj:4oiq
• PDBsum: 4oiq
• PubMed: 24755292
• UniProt: Q8RQE9|RPOB_THET8 DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)