Structure of PDB 4oau Chain C

Receptor sequence

>4oauC (length=692) Species: 9606 (Homo sapiens)

DNHLLIKAVQNEDVDLVQQLLEGGANVNFQEEEGGWTPLHNAVQMSREDI
VELLLRHGADPVLRKKNGATPFILAAIAGSVKLLKLFLSKGADVNECDFY
GFTAFMEAAVYGKVKALKFLYKRGANVNLRRKTKEDQERLRKGGATALMD
AAEKGHVEVLKILLDEMGADVNACDNMGRNALIHALLSSDDSDVEAITHL
LLDHGADVNVRGERGKTPLILAVEKKHLGLVQRLLEQEHIEINDTDSDGK
TALLLAVELKLKKIAELLCKRGASTDCGDLVMTARRNYDHSLVKVLLSHG
AKEDFHPPAEDWKPQSSHWGAALKDLHRIYRPMIGKLKFFIDEKYKIADT
SEGGIYLGFYEKQEVAVKTFCEGSPRAQREVSCLQSSRENSHLVTFYGSE
SHRGHLFVCVTLCEQTLEACLDVHRGEDVENEEDEFARNVLSSIFKAVQE
LHLSCGYTHQDLQPQNILIDSKKAAHLADFDKSIKWAGDPQEVKRDLEDL
GRLVLYVVKKGSISFEDLKAQSNEEVVQLSPDEETKDLIHRLFHPGEHVR
DCLSDLLGHPFFWTWESRYRTLRNVGNESDIKTRKSESEILRLLQPEHSK
SFDKWTTKINECVMKKMNKFYEKRGNFYQNTVGDLLKFIRNLGENIDEEK
HKKMKLKIGDPSLYFQKTFPDLVIYVYTKLQNTEYRKHFPQT

3D structure

• PDB: 4oau Structure of human RNase L reveals the basis for regulated RNA decay in the IFN response.
• Chain: C
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 3.1.26.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	E55 E57 G58 W60 Q68 K89 Y124 F126 E131 Y135 R155	E31 E33 G34 W36 Q44 K65 Y100 F102 E107 Y111 R131
BS02	ADP	C	I371 A372 T374 I379 A390 K392 T435 C437 T440 Q489 L492 D503 D505	I347 A348 T350 I355 A366 K368 T411 C413 T416 Q465 L468 D479 D481
BS03	MG	C	N490 D503	N466 D479
BS04	MG	C	D503 D505	D479 D481

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004540 RNA nuclease activity
• GO:0004672 protein kinase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0019843 rRNA binding
• GO:0043021 ribonucleoprotein complex binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006364 rRNA processing
• GO:0006396 RNA processing
• GO:0006397 mRNA processing
• GO:0006468 protein phosphorylation
• GO:0043488 regulation of mRNA stability
• GO:0045071 negative regulation of viral genome replication
• GO:0045444 fat cell differentiation
• GO:0045944 positive regulation of transcription by RNA polymerase II
• GO:0046326 positive regulation of D-glucose import
• GO:0051607 defense response to virus

Cellular Component

• GO:0005575 cellular_component
• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix
• GO:0005829 cytosol
• GO:0016363 nuclear matrix

External links

• PDB: RCSB:4oau, PDBe:4oau, PDBj:4oau
• PDBsum: 4oau
• PubMed: 24578532
• UniProt: Q05823|RN5A_HUMAN 2-5A-dependent ribonuclease (Gene Name=RNASEL)