Structure of PDB 4mim Chain C

Receptor sequence

>4mimC (length=597) Species: 347834 (Rhizobium etli CFN 42)

DRATKLLTYLADVTVNGHPEAKDRPKPLENAARPVVPYANGNGVKDGTKQ
LLDTLGPKKFGEWMRNEKRVLLTDTTMRDGHQSLLATRMRTYDIARIAGT
YSHALPNLLSLECWGGATFDVSMRFLTEDPWERLALIREGAPNLLLQMLL
RGANGVGYTNYPDNVVKYFVRQAAKGGIDLFRVFDCLNWVENMRVSMDAI
AEENKLCEAAICYTGDILNSARPKYDLKYYTNLAVELEKAGAHIIAVKDM
AGLLKPAAAKVLFKALREATGLPIHFHTHDTSGIAAATVLAAVEAGVDAV
DAAMDALSGNTSQPCLGSIVEALSGSERDPGLDPAWIRRISFYWEAVRNQ
YAAFESDLKGPASEVYLHEMPGGQFTNLKEQARSLGLETRWHQVAQAYAD
ANQMFGDIVKVTPSSKVVGDMALMMVSQDLTVADVVSPDREVSFPESVVS
MLKGDLGQPPSGWPEALQKKALKGEKPYTVRPGSLLKEADLDAERKVIEK
KLEREVSDFEFASYLMYPKVFTDFALASDTYGPVSVLPTPAYFYGLADGE
ELFADIEKGKTLVIVNQAVSATDSQGMVTVFFELNGQPRRIKVPDRA

3D structure

• PDB: 4mim Insights into the carboxyltransferase reaction of pyruvate carboxylase from the structures of bound product and intermediate analogs.
• Chain: C
• Resolution: 2.65 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D549 D655 K718 H747 H749 T882
• Catalytic site (residue number reindexed from 1): D79 D185 K248 H277 H279 T412
• Enzyme Commision number: 6.4.1.1: pyruvate carboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	C	D549 K718 H747 H749	D79 K248 H277 H279
BS02	BPV	C	R548 Q552 L619 R621	R78 Q82 L149 R151
BS03	MG	C	M534 R535 E537 D768	M64 R65 E67 D298

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004736 pyruvate carboxylase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006090 pyruvate metabolic process
• GO:0006094 gluconeogenesis

External links

• PDB: RCSB:4mim, PDBe:4mim, PDBj:4mim
• PDBsum: 4mim
• PubMed: 24157795
• UniProt: Q2K340