Structure of PDB 4m6v Chain C

Receptor sequence

>4m6vC (length=596) Species: 347834 (Rhizobium etli CFN 42)

DRATKLLTYLADVTVNGHPEAKDRPKPLENAARPVVPYAGNGVKDGTKQL
LDTLGPKKFGEWMRNEKRVLLTDTTMRDGHQSLLATRMRTYDIARIAGTY
SHALPNLLSLECWGGATFDVSMRFLTEDPWERLALIREGAPNLLLQMLLR
GANGVGYTNYPDNVVKYFVRQAAKGGIDLFRVFDCLNWVENMRVSMDAIA
EENKLCEAAICYTGDILNSARPKYDLKYYTNLAVELEKAGAHIIAVKDMA
GLLKPAAAKVLFKALREATGLPIHFHTHDTSGIAAATVLAAVEAGVDAVD
AAMDALSGNTSQPCLGSIVEALSGSERDPGLDPAWIRRISFYWEAVRNQY
AAFESDLKGPASEVYLHEMPGGQFTNLKEQARSLGLETRWHQVAQAYADA
NQMFGDIVKVTPSSKVVGDMALMMVSQDLTVADVVSPDREVSFPESVVSM
LKGDLGQPPSGWPEALQKKALKGEKPYTVRPGSLLKEADLDAERKVIEKK
LEREVSDFEFASYLMYPKVFTDFALASDTYGPVSVLPTPAYFYGLADGEE
LFADIEKGKTLVIVNQAVSATDSQGMVTVFFELNGQPRRIKVPDRA

3D structure

• PDB: 4m6v The role of biotin and oxamate in the carboxyltransferase reaction of pyruvate carboxylase.
• Chain: C
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D549 D655 K718 H747 H749 T882
• Catalytic site (residue number reindexed from 1): D78 D184 K247 H276 H278 T411
• Enzyme Commision number: 6.4.1.1: pyruvate carboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	C	D549 K718 H747 H749	D78 K247 H276 H278
BS02	MG	C	M534 R535 E537 D768	M63 R64 E66 D297
BS03	PYR	C	Q552 L619 R621 T882	Q81 L148 R150 T411
BS04	BYT	C	Y479 D482 G487 H488 P489 R594 F595 T597 Y1001 R1066	Y9 D12 G17 H18 P19 R123 F124 T126 Y530 R595
BS05	BYT	C	E490 R494 A556 F824 T846 E850	E20 R24 A85 F353 T375 E379

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004736 pyruvate carboxylase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006090 pyruvate metabolic process
• GO:0006094 gluconeogenesis

External links

• PDB: RCSB:4m6v, PDBe:4m6v, PDBj:4m6v
• PDBsum: 4m6v
• PubMed: 25157442
• UniProt: Q2K340