Structure of PDB 4kn4 Chain C

Receptor sequence
>4kn4C (length=1335) Species: 562 (Escherichia coli) [Search protein sequence]
KKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVF
PIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYER
EAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFF
DSDKGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPAT
IILRALNYTTEQILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEA
NGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKDYIDESTG
ELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTN
DRLSALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNR
SLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVG
EMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFG
SSQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGR
VCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLS
AIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVST
QQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERA
VAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYT
RSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNMRVAFMPW
NGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPNV
GEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFG
EKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAK
KDLSEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEK
QNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVK
RRIQPGDKMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRM
NIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQ
KVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDLPTS
GQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQ
PLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKN
IVDGNHQMEPGMPESFNVLLKEIRSLGINIELEDE
3D structure
PDB4kn4 X-ray Crystal Structures of the Escherichia coli RNA Polymerase in Complex with Benzoxazinorifamycins.
ChainC
Resolution3.965 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 1RL C Q513 F514 R529 S531 L533 R540 I572 Q506 F507 R522 S524 L526 R533 I565 PDBbind-CN: -logKd/Ki=7.24,IC50=57nM
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0016779 nucleotidyltransferase activity
GO:0032549 ribonucleoside binding
GO:0034062 5'-3' RNA polymerase activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006879 intracellular iron ion homeostasis
GO:0009408 response to heat
GO:0031564 transcription antitermination
GO:0032784 regulation of DNA-templated transcription elongation
GO:0036460 cellular response to cell envelope stress
GO:0042128 nitrate assimilation
GO:0044780 bacterial-type flagellum assembly
GO:0046677 response to antibiotic
GO:0048870 cell motility
GO:0071973 bacterial-type flagellum-dependent cell motility
GO:0090605 submerged biofilm formation
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0000428 DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0008023 transcription elongation factor complex
GO:0016020 membrane

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Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:4kn4, PDBe:4kn4, PDBj:4kn4
PDBsum4kn4
PubMed23679862
UniProtP0A8V2|RPOB_ECOLI DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)

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