Structure of PDB 4juo Chain C

Receptor sequence

>4juoC (length=546) Species: 170187 (Streptococcus pneumoniae TIGR4)

AVRKRPGMYIGSTDGAGLHHLVWEIVDNAVDEALSDRIDVTINKDGSLTV
QDHTVEVIFTILHAGGKFLHGVGSSVVNALSSWLEVEITRDGAVYKQRFE
NGGKPVTTLKKIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNES
AFLLKNVTLSLTDKRTDEAIEFHYEVQDFVSYLNEDKEILTPVLYFEGED
NGFQVEVALQYNDGFSDNILSFVNNVRTKDGGTHETGLKSAITKVMNDYA
RKTGLLKEKEGSDYREGLAAVLSILVPEEHLQFEGQTKDKLGSPLARPVV
DGIVADKLTFFLMENGELASNLIRKAIKARDAREAARKARLTPAQSKNPA
KNELYLVEGDSAGGSAKQGRDRKFQAILPLRGKVINTAKAKMADILKNEE
INTMIYTIGAGVGADFSIEDANYDKIIIMTDADTDGAHIQTLLLTFFYRY
MRPLVEAGHVYIAPPLYKAYAWTDGELEELRKLQRYKGLGEMNADQLWET
TMNPETRTLIRVTIEDLARAERRVNVLMGDKVEPRRKWIEDNVKFT

3D structure

• PDB: 4juo Structure of an 'open' clamp type II topoisomerase-DNA complex provides a mechanism for DNA capture and transport.
• Chain: C
• Resolution: 6.53 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	N461 K464 H513	N386 K389 H438
BS02	dna	C	G457 K458	G382 K383
BS03	dna	C	I460 N461 K464 V626 R629	I385 N386 K389 V532 R535
BS04	MG	C	D506 D508	D431 D433

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003916 DNA topoisomerase activity
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006265 DNA topological change
• GO:0007059 chromosome segregation

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:4juo, PDBe:4juo, PDBj:4juo
• PDBsum: 4juo
• PubMed: 23965305
• UniProt: Q59961|PARE_STRPN DNA topoisomerase 4 subunit B (Gene Name=parE)