Structure of PDB 4hqj Chain C

Receptor sequence

>4hqjC (length=987) Species: 9823 (Sus scrofa)

KEVSMDDHKLSLDELHRKYGTDLSRGLTPARAAEILARDGPNALTPPPTT
PEWVKFCRQLFGGFSMLLWIGAILCFLAYGIQAATEEEPQNDNLYLGVVL
SAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRNGEKMSINAEEVV
VGDLVEVKGGDRIPADLRIISANGCKVDNSSLTGESEPQTRSPDFTNENP
LETRNIAFFSTNCVEGTARGIVVYTGDRTVMGRIATLASGLEGGQTPIAA
EIEHFIHIITGVAVFLGVSFFILSLILEYTWLEAVIFLIGIIVANVPEGL
LATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRM
TVAHMWSDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQAN
QENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYTKIVEIPFNSTN
KYQLSIHKNPNTAEPRHLLVMKGAPERILDRCSSILIHGKEQPLDEELKD
AFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPLDNLCFV
GLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISE
GNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDILKYHT
EIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPASKKADIGVAMGI
AGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPE
ITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQP
RNPKTDKLVNEQLISMAYGQIGMIQALGGFFTYFVILAENGFLPIHLLGL
RVNWDDRWINDVEDSYGQQWTYEQRKIVEFTCHTPFFVTIVVVQWADLVI
CKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMGVALRMYPLKPT
WWFCAFPYSLLIFVYDEVRKLIIRRRPGGWVEKETYY

3D structure

• PDB: 4hqj Crystal structure of Na+, K(+)-ATPase in the Na(+)-bound state.
• Chain: C
• Resolution: 4.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D710 D714
• Catalytic site (residue number reindexed from 1): D681 D685
• Enzyme Commision number: 7.2.2.13: Na(+)/K(+)-exchanging ATPase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	C	F949 E953 A959 Y963 P975 K977	F920 E924 A930 Y934 P946 K948
BS02	ADP	C	F475 S477 Q482 G502 A503 L546 G611 D612 H613 R685	F446 S448 Q453 G473 A474 L517 G582 D583 H584 R656
BS03	ALF	C	D369 K370 T371 T610 G611	D340 K341 T342 T581 G582
BS04	MG	C	D369 T371 D710	D340 T342 D681
BS05	CLR	C	W924 W981	W895 W952
BS06	CLR	C	F860 T979	F831 T950

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0005215 transporter activity
• GO:0005391 P-type sodium:potassium-exchanging transporter activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008556 P-type potassium transmembrane transporter activity
• GO:0016887 ATP hydrolysis activity
• GO:0030955 potassium ion binding
• GO:0031402 sodium ion binding
• GO:0046872 metal ion binding
• GO:0051117 ATPase binding

Biological Process

• GO:0002028 regulation of sodium ion transport
• GO:0006813 potassium ion transport
• GO:0006814 sodium ion transport
• GO:0006883 intracellular sodium ion homeostasis
• GO:0010248 establishment or maintenance of transmembrane electrochemical gradient
• GO:0030007 intracellular potassium ion homeostasis
• GO:0036376 sodium ion export across plasma membrane
• GO:0055085 transmembrane transport
• GO:0086009 membrane repolarization
• GO:1902600 proton transmembrane transport
• GO:1990573 potassium ion import across plasma membrane

Cellular Component

• GO:0005886 plasma membrane
• GO:0005890 sodium:potassium-exchanging ATPase complex
• GO:0016020 membrane
• GO:0016323 basolateral plasma membrane
• GO:0030424 axon
• GO:0042383 sarcolemma
• GO:0042470 melanosome
• GO:0042995 cell projection

External links

• PDB: RCSB:4hqj, PDBe:4hqj, PDBj:4hqj
• PDBsum: 4hqj
• PubMed: 24051246
• UniProt: P05024|AT1A1_PIG Sodium/potassium-transporting ATPase subunit alpha-1 (Gene Name=ATP1A1)