Structure of PDB 4h49 Chain C

Receptor sequence

>4h49C (length=156) Species: 9606 (Homo sapiens)

VWRKHYITYRINNYTPDMNREDVDYAIRKAFQVWSNVTPLKFSKINTGMA
DILVVFARGAHGDDHAFDGKGGILAHAFGPGSGIGGDAHFDEDEFWTTHS
GGTNLFLTAVHEIGHSLGLGHSSDPKAVMFPTYKYVDINTFRLSADDIRG
IQSLYG

3D structure

• PDB: 4h49 Crystallization of bi-functional ligand protein complexes.
• Chain: C
• Resolution: 2.16 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H218 E219 H222 H228
• Catalytic site (residue number reindexed from 1): H111 E112 H115 H121
• Enzyme Commision number: 3.4.24.65: macrophage elastase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	C	H218 H222 H228	H111 H115 H121
BS02	ZN	C	H168 D170 H183 H196	H61 D63 H76 H89
BS03	CA	C	D158 G190 G192 D194	D51 G83 G85 D87
BS04	CA	C	D124 E199 E201	D17 E92 E94
BS05	CA	C	D175 G176 G178 I180 D198 E201	D68 G69 G71 I73 D91 E94
BS06	L29	C	I180 L181 A182 L214 H218 E219 H222 H228 V235 P238 T239 Y240	I73 L74 A75 L107 H111 E112 H115 H121 V128 P131 T132 Y133

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0031012 extracellular matrix

External links

• PDB: RCSB:4h49, PDBe:4h49, PDBj:4h49
• PDBsum: 4h49
• PubMed: 23567804
• UniProt: P39900|MMP12_HUMAN Macrophage metalloelastase (Gene Name=MMP12)