Structure of PDB 4g5q Chain C

Receptor sequence

>4g5qC (length=316) Species: 9606 (Homo sapiens) [Search protein sequence]

AREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQS
IIAIIRAMGRLKIDFGDSARADDARQLFVLAFMTAELAGVIKRLWKDSGV
QACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVE
THFTFKDLHFKMFDVGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLADE
EMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYP
EYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDA
VTDVIIKNNLKDCGLF

3D structure

PDB

4g5q Crystal Structures of the scaffolding protein LGN reveal the general mechanism by which GoLoco binding motifs inhibit the release of GDP from Galphai subunits in G-coupled heterotrimeric proteins

Chain

Resolution

2.9 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	E43 T48 R178 D200 Q204
Catalytic site (residue number reindexed from 1)	E13 T18 R142 D164 Q167
Enzyme Commision number	?

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	peptide	C	K257 W258	K219 W220
BS02	peptide	C	L39 G40 E43 S47 Q79 Q147 L148 N149 R178 V179 K180 Q204 W211 F215 L249 S252 N256	L9 G10 E13 S17 Q49 Q111 L112 N113 R142 V143 K144 Q167 W174 F178 L211 S214 N218
BS03	GDP	C	G42 E43 S44 G45 K46 S47 T48 S151 R176 N269 K270 D272 L273 C325 A326	G12 E13 S14 G15 K16 S17 T18 S115 R140 N231 K232 D234 L235 C287 A288

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0001664	G protein-coupled receptor binding
GO:0003924	GTPase activity
GO:0003925	G protein activity
GO:0005515	protein binding
GO:0005525	GTP binding
GO:0010854	adenylate cyclase regulator activity
GO:0016787	hydrolase activity
GO:0019001	guanyl nucleotide binding
GO:0019003	GDP binding
GO:0031683	G-protein beta/gamma-subunit complex binding
GO:0031749	D2 dopamine receptor binding
GO:0031821	G protein-coupled serotonin receptor binding
GO:0046872	metal ion binding

	Biological Process
GO:0007165	signal transduction
GO:0007186	G protein-coupled receptor signaling pathway
GO:0007188	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
GO:0007193	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
GO:0007198	adenylate cyclase-inhibiting serotonin receptor signaling pathway
GO:0034695	response to prostaglandin E
GO:0043434	response to peptide hormone
GO:0043949	regulation of cAMP-mediated signaling
GO:0045542	positive regulation of cholesterol biosynthetic process
GO:0051301	cell division
GO:0060236	regulation of mitotic spindle organization
GO:0072678	T cell migration
GO:1904322	cellular response to forskolin
GO:1904778	positive regulation of protein localization to cell cortex

	Cellular Component
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005730	nucleolus
GO:0005737	cytoplasm
GO:0005765	lysosomal membrane
GO:0005813	centrosome
GO:0005829	cytosol
GO:0005834	heterotrimeric G-protein complex
GO:0005856	cytoskeleton
GO:0005886	plasma membrane
GO:0005938	cell cortex
GO:0030496	midbody
GO:0070062	extracellular exosome

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:4g5q, PDBe:4g5q, PDBj:4g5q
PDBsum	4g5q
PubMed
UniProt	P63096\|GNAI1_HUMAN Guanine nucleotide-binding protein G(i) subunit alpha-1 (Gene Name=GNAI1)

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