Structure of PDB 4eo4 Chain C

Receptor sequence
>4eo4C (length=416) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
ATMTSMVSQRQDLFMTDPLSPGSMFFLPNGAKIFNKLIEFMKLQQKFKFG
FNEVVTPLIYKKTLWEKSGHWENYADDMFKVETTEKEEYGLKPMNCPGHC
LIFGKKDRSYNELPLRFSDFSPLHRNEASGALSGLTRLRKFHQDDGHIFC
TPSQVKSEIFNSLKLIDIVYNKIFPSNYFINFSTRPDHFIGDLKVWNHAE
QVLKEILEESGKPWKLNPGDGAFYGPKLDIMVTDHLRKTHQVATIQLDFQ
LPERFDLKFKDQDNSYKRPIMIHRATFGSIERFMALLIDSNEGRWPFWLN
PYQAVIIPVNTKNVQQLDMCTALQKKLRNELEADDMEPVPLNDWHFNVDL
DIRNEPVGYRIKSAILKNYSYLIIVGDEEVQLQKYNIRERDNRKSFEKLT
MSQIWEKFIELEKNYK
3D structure
PDB4eo4 The mechanism of pre-transfer editing in yeast mitochondrial threonyl-tRNA synthetase.
ChainC
Resolution2.87 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) C133 R162 Q180 D182 H184 K273 H319
Catalytic site (residue number reindexed from 1) C96 R125 Q143 D145 H147 K227 H273
Enzyme Commision number 6.1.1.3: threonine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SSA C M131 R162 T173 R174 L175 F178 Q180 Q287 V288 G324 S325 R328 M94 R125 T136 R137 L138 F141 Q143 Q241 V242 G278 S279 R282
BS02 SSA C V403 K408 I411 R434 R439 V357 K362 I365 R388 R393
BS03 ZN C C133 H184 H319 C96 H147 H273
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004829 threonine-tRNA ligase activity
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0140101 catalytic activity, acting on a tRNA
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006435 threonyl-tRNA aminoacylation
GO:0070159 mitochondrial threonyl-tRNA aminoacylation
Cellular Component
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4eo4, PDBe:4eo4, PDBj:4eo4
PDBsum4eo4
PubMed22773845
UniProtP07236|SYTM_YEAST Threonine--tRNA ligase, mitochondrial (Gene Name=MST1)

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