Structure of PDB 4c2t Chain C

Receptor sequence

>4c2tC (length=651) Species: 1299 (Deinococcus radiodurans)

TSSAGPDLLQALNPTQAQAADHFTGPALVIAGAGSGKTRTLIYRIAHLIG
HYGVHPGEILAVTFTNKAAAEMRERAGHLVPGAGDLWMSTFHSAGVRILR
TYGEHIGLRRGFVIYDDDDQLDIIKEVMGSIPGIGAETQPRVIRGIIDRA
KSNLWTPDDLDRSREPFISGLPRDAAAEAYRRYEVRKKGQNAIDFGDLIT
ETVRLFKEVPGVLDKVQNKAKFIHVDEYQDTNRAQYELTRLLASRDRNLL
VVGDPDQSIYKFRGADIQNILDFQKDYPDAKVYMLEHNYRSSARVLEAAN
KLIENNTERLDKTLKPVKEAGQPVTFHRATDHRAEGDYVADWLTRLHGEG
RAWSEMAILYRTNAQSRVIEESLRRVQIPRIVGGVGFYDRREIRDILAYA
RLALNPADDVALRRIIGRPRRGIGDTALQKLMEWARTHHTSVLTACANAA
EQNILDRGAHKATEFAGLMEAMSEAADNYEPAAFLRFVMETSGYLDLLRQ
EEGQVRLENLEELVSAAEEWSQDEANSIADFLDDAALLSSVDDMRTKAEP
EDAVTLMTLHNAKGLEFPVVFIVGVEQGLLPSKGAIAEGPSGIEEERRLF
YVGITRAMERLLMTAAQNRMQFGKTNAAEDSAFLEDIEGLFDTVDPYGQP
I

3D structure

• PDB: 4c2t Structural and Mechanistic Insight Into DNA Unwinding by Deinococcus Radiodurans Uvrd.
• Chain: C
• Resolution: 3.997 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K38 T39 D227 E228 Q258 R617
• Catalytic site (residue number reindexed from 1): K37 T38 D226 E227 Q257 R606
• Enzyme Commision number: 5.6.2.4: DNA 3'-5' helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	H93 Q140 R142 Y261 F263 R362 T363 R422 G424 I425 G426 R459 S546 S547 H571 S593 F633	H92 Q139 R141 Y260 F262 R361 T362 R420 G422 I423 G424 R457 S539 S540 H560 S582 F622	PDBbind-CN: Kd=0.36uM
BS02	dna	C	F633 G634	F622 G623	PDBbind-CN: Kd=0.36uM
BS03	MG	C	T39 D227	T38 D226
BS04	ANP	C	Q17 G35 S36 G37 K38 T39 R40 Q258 Y290 R291 E577 R617	Q16 G34 S35 G36 K37 T38 R39 Q257 Y289 R290 E566 R606

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003678 DNA helicase activity
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016853 isomerase activity
• GO:0016887 ATP hydrolysis activity
• GO:0043138 3'-5' DNA helicase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0000725 recombinational repair
• GO:0032508 DNA duplex unwinding

Cellular Component

• GO:0005829 cytosol
• GO:0033202 DNA helicase complex

External links

• PDB: RCSB:4c2t, PDBe:4c2t, PDBj:4c2t
• PDBsum: 4c2t
• PubMed: 24143224
• UniProt: Q9RTI9