Structure of PDB 3zq4 Chain C

Receptor sequence

>3zq4C (length=542) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

NDQTAVFALGGLGEIGKNTYAVQFQDEIVLIDAGIKFPEDELLGIDYVIP
DYTYLVKNEDKIKGLFITHGHEDHIGGIPYLLRQVNIPVYGGKLAIGLLR
NKLEEHGLLRQTKLNIIGEDDIVKFRKTAVSFFRTTHSIPDSYGIVVKTP
PGNIVHTGDFKFDFTPVGEPANLTKMAEIGKEGVLCLLSDSTNSENPEFT
MSERRVGESIHDIFRKVDGRIIFATFASNIHRLQQVIEAAVQNGRKVAVF
GRSMESAIEIGQTLGYINCPKNTFIEHNEINRMPANKVTILCTGSQGEPM
AALSRIANGTHRQISINPGDTVVFSSSPIPGNTISVSRTINQLYRAGAEV
IHGPLNDIHTSGHGGQEEQKLMLRLIKPKFFMPIHGEYRMQKMHVKLATD
CGIPEENCFIMDNGEVLALKGDEASVAGKIPSGSVYIDGSGIGDIGNIVL
RDRRILSEEGLVIVVVSIDMDDFKISAGPDLISRGFVINDAQELISNHLQ
KVMERKTTQWSEIKNEITDTLAPFLYEKTKRRPMILPIIMEV

3D structure

• PDB: 3zq4 Unusual, Dual Endo- and Exonuclease Activity in the Degradosome Explained by Crystal Structure Analysis of Rnase J1.
• Chain: C
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	C	D78 H79 D164 H390	D73 H74 D159 H385
BS02	ZN	C	H74 H76 H142	H69 H71 H137
BS03	CA	C	G49 D443	G44 D438

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0004534 5'-3' RNA exonuclease activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006364 rRNA processing
• GO:0006396 RNA processing
• GO:0006397 mRNA processing

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3zq4, PDBe:3zq4, PDBj:3zq4
• PDBsum: 3zq4
• PubMed: 21893285
• UniProt: Q45493|RNJ1_BACSU Ribonuclease J1 (Gene Name=rnjA)