Structure of PDB 3wiv Chain C

Receptor sequence

>3wivC (length=397) Species: 69014 (Thermococcus kodakarensis KOD1)

EQNTIRVIVSVDKAKFNPHEVLGIGGHIVYQFKLIPAVVVDVPANAVGKL
KKMPGVEKVEFDHQAVLLGKPSWLGGGSTQPAQTIPWGIERVKAPSVWSI
TDGSVSVIQVAVLDTGVDYDHPDLAANIAWCVSTLRGKVSTKLRDCADQN
GHGTHVIGTIAALNNDIGVVGVAPGVQIYSVRVLDARGSGSYSDIAIGIE
QAILGPDGVADKDGDGIIAGDPDDDAAEVISMSLGGPADDSYLYDMIIQA
YNAGIVIVAASGNEGAPSPSYPAAYPEVIAVGAIDSNDNIASFSNRQPEV
SAPGVDILSTYPDDSYETLMGTAMATPHVSGVVALIQAAYYQKYGKILPV
GTFDAISKNTVRGILHITADDLGPTGWDADYGYGVVRAALAVQAALG

3D structure

• PDB: 3wiv Requirement of the insertion sequence for activation of Tk-subtilisin
• Chain: C
• Resolution: 1.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D115 H153 N264 A324
• Catalytic site (residue number reindexed from 1): D114 H152 N263 A323
• Enzyme Commision number: 3.4.21.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	C	Q84 D124 L164 N166 I168 V170	Q83 D123 L163 N165 I167 V169
BS02	CA	C	L205 D208 V210 D226	L204 D207 V209 D225
BS03	CA	C	D212 D214 D216 I218 D222 D225	D211 D213 D215 I217 D221 D224
BS04	CA	C	D214 D216 D222 D224	D213 D215 D221 D223
BS05	CA	C	V108 Q110 A227 E229	V107 Q109 A226 E228
BS06	CA	C	D372 L373 P375 G377 D379	D371 L372 P374 G376 D378

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0008236 serine-type peptidase activity
• GO:0042802 identical protein binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:3wiv, PDBe:3wiv, PDBj:3wiv
• PDBsum: 3wiv
• UniProt: P58502|TKSU_THEKO Tk-subtilisin (Gene Name=TK1675)