Structure of PDB 3wiu Chain C

Receptor sequence

>3wiuC (length=394) Species: 69014 (Thermococcus kodakarensis KOD1)

NTIRVIVSVDKAKFNPHEVLGIGGHIVYQFKLIPAVVVDVPANAVGKLKK
MPGVEKVEFDHQAVLLGKPSWLGGSTQPAQTIPWGIERVKAPSVWSITDG
SVSVIQVAVLDTGVDYDHPDLAANIAWCVSTLRGKVSTKLRDCADQNGHG
THVIGTIAALNNDIGVVGVAPGVQIYSVRVLDARGSGSYSDIAIGIEQAI
LGPDGVADKDGDGIIAGDPDDDAAEVISMSLGGPADDSYLYDMIIQAYNA
GIVIVAASGNEGAPSPSYPAAYPEVIAVGAIDSNDNIASFSNRQPEVSAP
GVDILSTYPDDSYETLMGTAMATPHVSGVVALIQAAYYQKYGKIAPVGTF
DDISKNTVRGILHITADDLGPTGWDADYGYGVVRAALAVQAALG

3D structure

• PDB: 3wiu Requirement of the insertion sequence for activation of Tk-subtilisin
• Chain: C
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D115 H153 N264 A324
• Catalytic site (residue number reindexed from 1): D111 H149 N260 A320
• Enzyme Commision number: 3.4.21.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	C	Q84 D124 L164 N166 I168 V170	Q80 D120 L160 N162 I164 V166
BS02	CA	C	L205 D208 V210 D226	L201 D204 V206 D222
BS03	CA	C	D212 D214 D216 I218 D222 D225	D208 D210 D212 I214 D218 D221
BS04	CA	C	D214 D216 D222 D224	D210 D212 D218 D220
BS05	CA	C	V108 Q110 A227 E229	V104 Q106 A223 E225
BS06	CA	C	D372 L373 P375 G377 D379	D368 L369 P371 G373 D375

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0008236 serine-type peptidase activity
• GO:0042802 identical protein binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:3wiu, PDBe:3wiu, PDBj:3wiu
• PDBsum: 3wiu
• UniProt: P58502|TKSU_THEKO Tk-subtilisin (Gene Name=TK1675)