Structure of PDB 3wgu Chain C

Receptor sequence

>3wguC (length=994) Species: 9823 (Sus scrofa)

RDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTPARAAEILARDGPNA
LTPPPTTPEWVKFCRQLFGGFSMLLWIGAILCFLAYGIQAATEEEPQNDN
LYLGVVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRNGEKMS
INAEEVVVGDLVEVKGGDRIPADLRIISANGCKVDNSSLTGESEPQTRSP
DFTNENPLETRNIAFFSTNCVEGTARGIVVYTGDRTVMGRIATLASGLEG
GQTPIAAEIEHFIHIITGVAVFLGVSFFILSLILEYTWLEAVIFLIGIIV
ANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTG
TLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCN
RAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYTKIVE
IPFNSTNKYQLSIHKNPNTAEPRHLLVMKGAPERILDRCSSILIHGKEQP
LDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFP
LDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAK
GVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLD
DILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKAD
IGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYT
LTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAES
DIMKRQPRNPKTDKLVNERLISMAYGQIGMIQALGGFFTYFVILAENGFL
PIHLLGLRVNWDDRWINDVEDSYGQQWTYEQRKIVEFTCHTAFFVSIVVV
QWADLVICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMGVALR
MYPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRRPGGWVEKETYY

3D structure

• PDB: 3wgu Crystal structure of a Na1-bound Na1,K1-ATPase preceding the E1P state
• Chain: C
• Resolution: 2.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D710 D714
• Catalytic site (residue number reindexed from 1): D688 D692
• Enzyme Commision number: 7.2.2.13: Na(+)/K(+)-exchanging ATPase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ALF	C	D369 T371 T610 G611 K691 N713	D347 T349 T588 G589 K669 N691
BS02	MG	C	D369 T371 D710	D347 T349 D688
BS03	ADP	C	T371 D443 E446 F475 S477 K480 Y481 Q482 K501 G502 A503 R544 L546 D612 R685	T349 D421 E424 F453 S455 K458 Y459 Q460 K479 G480 A481 R522 L524 D590 R663
BS04	CLR	C	F860 P986	F838 P964
BS05	CLR	C	W924 Y963 W981 F985	W902 Y941 W959 F963

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0005215 transporter activity
• GO:0005391 P-type sodium:potassium-exchanging transporter activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008556 P-type potassium transmembrane transporter activity
• GO:0016887 ATP hydrolysis activity
• GO:0030955 potassium ion binding
• GO:0031402 sodium ion binding
• GO:0046872 metal ion binding
• GO:0051117 ATPase binding

Biological Process

• GO:0002028 regulation of sodium ion transport
• GO:0006813 potassium ion transport
• GO:0006814 sodium ion transport
• GO:0006883 intracellular sodium ion homeostasis
• GO:0010248 establishment or maintenance of transmembrane electrochemical gradient
• GO:0030007 intracellular potassium ion homeostasis
• GO:0036376 sodium ion export across plasma membrane
• GO:0055085 transmembrane transport
• GO:0086009 membrane repolarization
• GO:1902600 proton transmembrane transport
• GO:1990573 potassium ion import across plasma membrane

Cellular Component

• GO:0005886 plasma membrane
• GO:0005890 sodium:potassium-exchanging ATPase complex
• GO:0016020 membrane
• GO:0016323 basolateral plasma membrane
• GO:0030424 axon
• GO:0042383 sarcolemma
• GO:0042470 melanosome
• GO:0042995 cell projection

External links

• PDB: RCSB:3wgu, PDBe:3wgu, PDBj:3wgu
• PDBsum: 3wgu
• UniProt: P05024|AT1A1_PIG Sodium/potassium-transporting ATPase subunit alpha-1 (Gene Name=ATP1A1)