Structure of PDB 3t0t Chain C

Receptor sequence
>3t0tC (length=583) Species: 282458 (Staphylococcus aureus subsp. aureus MRSA252) [Search protein sequence]
MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHKGRIDTI
RKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGT
PEKFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSG
ELKNKKGVNLPGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDV
LEIREILEEQKANISVFPKIENQEGIDNIEEILEVSDGLMVARGDMGVEI
PPEKVPMVQKDLIRQCNKLGKPVITATQMLDSMQRNPRATRAEASDVANA
IYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSAEAAQDYKKLLSDRTKL
VETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYRPHSDIIAV
TPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVTNGDLI
IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDL
EGKDLSDKVIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIP
TVVGVEKAVKNISNNVLVTIDAAQGKIFEGYAN
3D structure
PDB3t0t Cheminformatics-driven discovery of selective, nanomolar inhibitors for staphylococcal pyruvate kinase.
ChainC
Resolution3.1 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R32 R73 K219 T277
Catalytic site (residue number reindexed from 1) R32 R73 K219 T277
Enzyme Commision number 2.7.1.40: pyruvate kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 I30 C T353 A358 I361 S362 H365 T353 A358 I361 S362 H365 BindingDB: IC50=91nM
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003824 catalytic activity
GO:0004743 pyruvate kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016772 transferase activity, transferring phosphorus-containing groups
GO:0030955 potassium ion binding
GO:0046872 metal ion binding
Biological Process
GO:0006096 glycolytic process
GO:0016310 phosphorylation
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3t0t, PDBe:3t0t, PDBj:3t0t
PDBsum3t0t
PubMed22066782
UniProtQ6GG09|KPYK_STAAR Pyruvate kinase (Gene Name=pyk)

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