Structure of PDB 3rt1 Chain C

Receptor sequence

>3rt1C (length=646) Species: 4932 (Saccharomyces cerevisiae)

SRDLQNHLLFETATEVANRVGGIYSVLKSKAPITVAQYKDHYHLIGPLNK
ATYQNEVDILDWKKPEAFSDEMRPVQHALQTMESRGVHFVYGRWLIEGAP
KVILFDLDSVRGYSNEWKGDLWSLVGIPSPENDFETNDAILLGYTVAWFL
GEVAHLDSQHAIVAHFHEWLAGVALPLCRKRRIDVVTIFTTHATLLGRYL
CASGSFDFYNCLESVDVDHEAGRFGIYHRYCIERAAAHSADVFTTVSQIT
AFEAEHLLKRKPDGILPNGLNVIKFQAFHEFQNLHALKKEKINDFVRGHF
HGCFDFDLDNTLYFFIAGRYEYKNKGADMFIEALARLNYRLKVSGSKKTV
VAFIVMPAKNNSFTVEALKGQAEVRALENTVHEVTTSIGKRIFDHAIRYP
HNGLTTELPTDLGELLKSSDKVMLKRRILALRRPEGQLPPIVTHNMVDDA
NDLILNKIRQVQLFNSPSDRVKMIFHPEFLNANNPILGLDYDEFVRGCHL
GVFPSYYEPWGYTPAECTVMGVPSITTNVSGFGSYMEDLIETNQAKDYGI
YIVDRRFKAPDESVEQLVDYMEEFVKKTRRQRINQRNATEALSDLLDWKR
MGLEYVKARQLALRRGYPDQFRELVGEELNDSNMDALAGGKKLKVA

3D structure

• PDB: 3rt1 Multiple Glycogen-binding Sites in Eukaryotic Glycogen Synthase Are Required for High Catalytic Efficiency toward Glycogen.
• Chain: C
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.11: glycogen(starch) synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	C	E117 W118 D121 R182	E116 W117 D120 R181
BS02	GLC	C	W149 R182	W148 R181
BS03	GLC	C	G152 E153	G151 E152
BS04	G6P	C	Q283 H286 K290 H500 R580 R583 R587	Q282 H285 K289 H499 R579 R582 R586

Gene Ontology

Molecular Function

• GO:0004373 alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity
• GO:0005515 protein binding
• GO:0016757 glycosyltransferase activity
• GO:0042802 identical protein binding
• GO:2001069 glycogen binding

Biological Process

• GO:0005978 glycogen biosynthetic process

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0042587 glycogen granule

External links

• PDB: RCSB:3rt1, PDBe:3rt1, PDBj:3rt1
• PDBsum: 3rt1
• PubMed: 21835915
• UniProt: P27472|GYS2_YEAST Glycogen [starch] synthase isoform 2 (Gene Name=GSY2)