Structure of PDB 3ho8 Chain C

Receptor sequence

>3ho8C (length=995) Species: 158878 (Staphylococcus aureus subsp. aureus Mu50)

QIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHRYKADESYL
VGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDERYIDNPKHIEVQV
IGDEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSPTLRQRICDAAIQLM
ENIKYVNAGTVEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQIL
VAAGADLFGEEINMPQQKDITTLGYAIQCRITTEDPLNDFMPDTGTIIAY
RSSGGFGVRLDAGDGFQGAEISPYYDSLLVKLSTHAISFKQAEEKMVRSL
REMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFIEETPELFDIQPSLDRG
TKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASFSGTKQLLD
EVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRAS
NAVGYKNYPDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEA
GKISEGTICYTGDILNPERSNIYTLEYYVKLAKELEREGFHILAIKDMAG
LLKPKAAYELIGELKSAVDLPIHLHTHDTSGNGLLTYKQAIDAGVDIIDT
AVASMSGLTSQPSANSLYYALNGFPRHLRTDIEGMESLSHYWSTVRTYYS
DFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFDEVKDMYRRVN
FLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVSFF
KGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQ
QGPVTEQDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETV
EIEIDKGKRLIIKLETISEPDENGNRTIYYAMNGQARRIYIKDEN

3D structure

• PDB: 3ho8 A Symmetrical Tetramer for S. aureus Pyruvate Carboxylase in Complex with Coenzyme A.
• Chain: C
• Resolution: 2.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K152 H244 R270 T309 E311 E324 N326 R328 E332 R377 D572 D678 K741 H771 H773 I794 S800 M801 T840 T908 S910 V922 N924 Q929
• Catalytic site (residue number reindexed from 1): K117 H145 R171 T210 E212 E224 N226 R228 E232 R280 D476 D582 K646 H675 H677 I698 S704 M705 T744 T810 S812 V824 N826 Q831
• Enzyme Commision number: 6.4.1.1: pyruvate carboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	COA	C	R77 Y78 A80 S83	R42 Y43 A45 S48
BS02	COA	C	R398 E449 R451 R453 S493 L494 R496 I1052 K1056 N1081 R1085	R301 E352 R354 R356 S396 L397 R399 I954 K958 N983 R987
BS03	MN	C	D572 H771 H773	D476 H675 H677
BS04	BTI	C	N506 G511 F512 P513 F618 K620	N409 G414 F415 P416 F522 K524

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004736 pyruvate carboxylase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006090 pyruvate metabolic process
• GO:0006094 gluconeogenesis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3ho8, PDBe:3ho8, PDBj:3ho8
• PDBsum: 3ho8
• PubMed: 19523900
• UniProt: A0A0H3JRU9|PYC_STAAM Pyruvate carboxylase (Gene Name=pycA)