Structure of PDB 3hfg Chain C

Receptor sequence
>3hfgC (length=254) Species: 9606 (Homo sapiens) [Search protein sequence]
NEEFRPEMLQGKKVIVTGASKGIGREMAYHLAKMGAHVVVTARSKETLQK
VVSHCLELGAASAHYIAGTMEDMTFAEQFVAQAGKLMGGLDMLILNHITN
TSLNLFHDDIHHVRKSMEVNFLSYVVLTVAALPMLKQSNGSIVVVSSLAG
KVAYPMVAAYSASKFALDGFFSSIRKEYSVSRVNVSITLCVLGLIDTETA
MKAVSGIAAPKEECALEIIKGGALRQEEVYYDSSLWTTLLIRNPSRKILE
FLYS
3D structure
PDB3hfg Efficacious 11beta-hydroxysteroid dehydrogenase type I inhibitors in the diet-induced obesity mouse model.
ChainC
Resolution2.3 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) S170 Y183 K187
Catalytic site (residue number reindexed from 1) S147 Y160 K164
Enzyme Commision number 1.1.1.146: 11beta-hydroxysteroid dehydrogenase.
1.1.1.201: 7beta-hydroxysteroid dehydrogenase (NADP(+)).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 17R C L126 L171 A172 Y177 Y183 G216 L217 T222 A226 L103 L148 A149 Y154 Y160 G193 L194 T199 A203 MOAD: ic50=10nM
BindingDB: IC50=26nM
BS02 NAP C G41 S43 K44 R66 S67 T92 M93 N119 I121 V168 S170 Y183 K187 L215 G216 I218 T220 T222 G18 S20 K21 R43 S44 T69 M70 N96 I98 V145 S147 Y160 K164 L192 G193 I195 T197 T199
Gene Ontology
Molecular Function
GO:0005496 steroid binding
GO:0016491 oxidoreductase activity
GO:0042803 protein homodimerization activity
GO:0047022 7-beta-hydroxysteroid dehydrogenase (NADP+) activity
GO:0050661 NADP binding
GO:0070524 11-beta-hydroxysteroid dehydrogenase (NADP+) activity
GO:0102196 cortisol dehydrogenase activity
Biological Process
GO:0006706 steroid catabolic process
GO:0008202 steroid metabolic process
GO:0030324 lung development
Cellular Component
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0043231 intracellular membrane-bounded organelle

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3hfg, PDBe:3hfg, PDBj:3hfg
PDBsum3hfg
PubMed19673466
UniProtP28845|DHI1_HUMAN 11-beta-hydroxysteroid dehydrogenase 1 (Gene Name=HSD11B1)

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