Structure of PDB 3h9u Chain C

Receptor sequence

>3h9uC (length=435) Species: 5691 (Trypanosoma brucei)

SMYKVRDISLAEWGRRELELAENEMPGLMELRREYGPSKPLKGAKIAGCL
HMTMQTAVLIETLVELGAEVRWASCNIFSTQDHAAAAIAKRGIPVFAWKG
ETEEEYMWCMKQTLKGFSGDGYPNMLLDDGGDLTNYVLDECELDGKIYGV
SEETTTGVKNLYKRLQRGKLTIPAMNVNDSVTKSKFDNLYGCRESLVDGI
KRATDVMIAGKTACVCGYGDVGKGCAAALRGFGARVVVTEVDPINALQAA
MEGYQVLLVEDVVEEAHIFVTTTGNDDIITSEHFPRMRDDAIVCNIGHFD
TEIQVAWLKANAKERVEVKPQVDRYTMANGRHIILLAEGRLVNLGCASGH
PSFVMSNSFCNQVLAQIELWTNRDTGKYPRGAKAQVYFLPKKLDEKVAAL
HLGKLGAKLTKLTPKQAEYINCPVDGPFKPDHYRY

3D structure

• PDB: 3h9u Crystal structure of S-adenosyl homocysteine hydrolase (SAHH) from Trypanosoma brucei
• Chain: C
• Resolution: 1.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H52 S75 S80 D130 E155 N180 K185 D189 N190 C194 H300 H352 S360 Q364
• Catalytic site (residue number reindexed from 1): H51 S74 S79 D129 E153 N178 K183 D187 N188 C192 H298 H350 S358 Q362
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	C	T156 T157 T158 N190 G221 D222 V223 E242 V243 N247 T275 G276 N277 I280 I298 G299 H300 N345 H352	T154 T155 T156 N188 G219 D220 V221 E240 V241 N245 T273 G274 N275 I278 I296 G297 H298 N343 H350
BS02	ADN	C	T54 Q56 L346 H352 M357	T53 Q55 L344 H350 M355
BS03	NAD	C	K431 Y435	K429 Y433

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:3h9u, PDBe:3h9u, PDBj:3h9u
• PDBsum: 3h9u
• UniProt: Q383X0