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Structure of PDB 3g5n Chain C

Receptor sequence

>3g5nC (length=452) Species: 9986 (Oryctolagus cuniculus)

SKGKLPPGPSPLPVLGNLLQMDRKGLLRSFLRLREKYGDVFTVYLGSRPV
VVLCGTDAIREALVDQAEAFSGRGKIAVVDPIFQGYGVIFANGERWRALR
RFSLATMRDKRSVEERIQEEARCLVEELRKSKGALLDNTLLFHSITSNII
CSIVFGKRFDYKDPVFLRLLDLFFQSFSLISSFSSQVFELFSGFLKYFPG
THRQIYRNLQEINTFIGQSVEKHRATLDPSNPRDFIDVYLLRMEHQNLIL
TVLSLFFAGTETTSTTLRYGFLLMLKYPHVTERVQKEIEQVIGSHRPPAL
DDRAKMPYTDAVIHEIQRLGDLIPFGVPHTVTKDTQFRGYVIPKNTEVFP
VLSSALHDPRYFETPNTFNPGHFLDANGALKRNEGFMPFSLGKRICLGEG
IARTELFLFFTTILQNFSIASPVPPEDIDLTPRESGVGNVPPSYQIRFLA
RH

3D structure

• PDB: 3g5n Crystal structures of cytochrome P450 2B4 in complex with the inhibitor 1-biphenyl-4-methyl-1H-imidazole: ligand-induced structural response through alpha-helical repositioning.
• Chain: C
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T302 F429 C436
• Catalytic site (residue number reindexed from 1): T262 F389 C396
• Enzyme Commision number: 1.14.14.1: unspecific monooxygenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	C	R98 W121 R125 F296 G299 T302 T303 I363 V367 H369 P428 F429 S430 R434 C436 L437 G438	R73 W96 R100 F256 G259 T262 T263 I323 V327 H329 P388 F389 S390 R394 C396 L397 G398
BS02	PB2	C	A298 G299 I363 P368	A258 G259 I323 P328	MOAD: ic50=0.035uM
BS03	PB2	C	L43 L51 Y69 L70 V477	L18 L26 Y44 L45 V437	MOAD: ic50=0.035uM
BS04	PB2	C	R73 E387	R48 E347	MOAD: ic50=0.035uM
BS05	CM5	C	R98 N117 L288	R73 N92 L248
BS06	PB2	C	F217 E218	F188 E189	MOAD: ic50=0.035uM
BS07	PB2	C	P228 T230	P199 T201	MOAD: ic50=0.035uM

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005506 iron ion binding
• GO:0008392 arachidonate epoxygenase activity
• GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
• GO:0016712 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0070330 aromatase activity

Biological Process

• GO:0006805 xenobiotic metabolic process
• GO:0019373 epoxygenase P450 pathway

Cellular Component

• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005789 endoplasmic reticulum membrane
• GO:0016020 membrane
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:3g5n, PDBe:3g5n, PDBj:3g5n
• PDBsum: 3g5n
• PubMed: 19397311
• UniProt: P00178|CP2B4_RABIT Cytochrome P450 2B4 (Gene Name=CYP2B4)

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