Structure of PDB 3e3j Chain C

Receptor sequence

>3e3jC (length=833) Species: 10760 (Escherichia phage T7)

KNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMFLI
TTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLA
CLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFKKNVKAFMQVVE
ADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVV
GQDSETIELAPEYAEAIAGISLMFQPCVVPPKPWTGITGGGYWANGRRPL
ALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKW
KHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSR
RISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLL
TLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAK
SPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQ
HFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQADAINGTDNEV
VTVTISEKVKLGTKALAGQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQV
LEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLK
SAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLM
FLGQFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHE
KYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFA
DQLHESQLDKMPALPAKGNLNLRDILESDFAFA

3D structure

• PDB: 3e3j The structure of a transcribing t7 RNA polymerase in transition from initiation to elongation
• Chain: C
• Resolution: 6.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	R96 G97 R99 R231 V237 D240 E242 R298 R423 Y427 T636 Y639 G640 S641 K642 R746 F755 R756 Q758 P759 T760 S776 N781 H784	R72 G73 R75 R194 V200 D203 E205 R254 R379 Y383 T586 Y589 G590 S591 K592 R696 F705 R706 Q708 P709 T710 S726 N731 H734
BS02	dna	C	K95 R96 G97 K98 P100 H211 G235 F644 R647 K704 L747 N748	K71 R72 G73 K74 P76 H174 G198 F594 R597 K654 L697 N698
BS03	rna	C	K389 A390 R425 S541 Y639 H784 I810 D812	K345 A346 R381 S497 Y589 H734 I760 D762

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0019083 viral transcription
• GO:0039695 DNA-templated viral transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:3e3j, PDBe:3e3j, PDBj:3e3j
• PDBsum: 3e3j
• PubMed: 18948533
• UniProt: P00573|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)