Structure of PDB 3cp8 Chain C

Receptor sequence

>3cp8C (length=611) Species: 194439 (Chlorobaculum tepidum TLS)

HMYDVIVVGAGHAGCEAALAVARGGLHCLLITSDLSAVARMSCNPAIGGV
AKGQITREIDALGGEMGKAIDATGIQFRMLNRSKGPAMHSPRAQADKTQY
SLYMRRIVEHEPNIDLLQDTVIGVSANSGKFSSVTVRSGRAIQAKAAILA
CGTFLNGLIHIGMDHFPGGRSTAEPPVEGLTESLASLGFSFGRLKTGTPP
RIDSRSVDYTIVTEQPGDVDPVPFSFSSTSVANRNLVSCYLTKTTEKTHD
ILRTGFDRSPLFTGCPSIEDKISRFPDKSSHHIFLEPEGTDTVEMYVNGF
STSLPEDIQIAGLRSIPGLEEAKMIRPGYAIEYDFFHPWQIRSTMETRPV
ENLFFAGQINGTSGYEEAAAQGLMAGINAVRKILGKELIVLGRDQAYIGV
LIDDLITKETKEPYRMFTSSAEHRLILRHDNADLRLRKIGYDCNLVSSDD
LHRTESIIKRVQHCLEVMKTAKVTPAEINTLLMNKGLQELKTPARALSLI
KRPGISLQDILEHSLSVRSAAEELCNDPRVAEQVQIEIKYEGYIKREQLV
ADRIARLDSLHIPDNFNYDSLNSLSSEGREKLLKHRPATIGQASRILGVS
PSDVSILMIRL

3D structure

• PDB: 3cp8 Crystal structures of the conserved tRNA-modifying enzyme GidA: implications for its interaction with MnmE and substrate
• Chain: C
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 1.-.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	C	G8 G10 H11 T31 S32 C42 K51 T119 V120 C150 G151 F153 V176 T195 G196 G365 Q366 G372 Y373 E374 A376	G9 G11 H12 T32 S33 C43 K52 T120 V121 C151 G152 F154 V177 T196 G197 G357 Q358 G364 Y365 E366 A368

Gene Ontology

Molecular Function

• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0002098 tRNA wobble uridine modification
• GO:0008033 tRNA processing
• GO:0030488 tRNA methylation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:3cp8, PDBe:3cp8, PDBj:3cp8
• PDBsum: 3cp8
• PubMed: 18565343
• UniProt: Q8KA85|MNMG_CHLTE tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (Gene Name=mnmG)