Structure of PDB 3ccj Chain C

Receptor sequence

>3ccjC (length=246) Species: 2238 (Haloarcula marismortui)

MQATIYDLDGNTDGEVDLPDVFETPVRSDLIGKAVRAAQANRKQDYGSDE
YAGLRTPAESFGSGRGQAHVPKQDGRARRVPQAVKGRSAHPPKTEKDRSL
DLNDKERQLAVRSALAATADADLVADRGHEFDRDEVPVVVSDDFEDLVKT
QEVVSLLEALDVHADIDRADETKIKAGQGSARGRKYRRPASILFVTSDEP
STAARNLAGADVATASEVNTEDLAPGGAPGRLTVFTESALAEVAER

3D structure

• PDB: 3ccj Mutations outside the anisomycin-binding site can make ribosomes drug-resistant.
• Chain: C
• Resolution: 3.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	R27 R36 A38 N41 R42 K43 Q44 Y46 G47 S48 D49 A52 R55 T56 P57 S63 G64 G66 Q67 A68 H69 V70 P71 Q73 D74 R76 A77 V80 P81 Q82 A83 V84 K85 R87 H90 P92 K93 E95 K96 D97 L100 D101 N103 D104 K105 E106 R107 L109 R127 K149 T150 R168 D170 K173 I174 K175 A176 G177 Q178 G179 R182 R184 Y186 R187 R188 P189 A190 T202 R205 N206 A213 E217 V218 N219 D222 P225 G226 R246	R27 R36 A38 N41 R42 K43 Q44 Y46 G47 S48 D49 A52 R55 T56 P57 S63 G64 G66 Q67 A68 H69 V70 P71 Q73 D74 R76 A77 V80 P81 Q82 A83 V84 K85 R87 H90 P92 K93 E95 K96 D97 L100 D101 N103 D104 K105 E106 R107 L109 R127 K149 T150 R168 D170 K173 I174 K175 A176 G177 Q178 G179 R182 R184 Y186 R187 R188 P189 A190 T202 R205 N206 A213 E217 V218 N219 D222 P225 G226 R246

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3ccj, PDBe:3ccj, PDBj:3ccj
• PDBsum: 3ccj
• PubMed: 18455733
• UniProt: P12735|RL4_HALMA Large ribosomal subunit protein uL4 (Gene Name=rpl4)