Structure of PDB 3ayx Chain C

Receptor sequence

>3ayxC (length=595) Species: 28885 (Hydrogenovibrio marinus)

SVLNTPNHYKMDNSGRRVVIDPVTRIEGHMRCEVNVDENNVIQNAVSTGT
MWRGLEVILRGRDPRDAWAFVERICGVCTGCHALASVRAVEDALDIKIPH
NATLIREIMAKTLQIHDHIVHFYHLHALDWVNPVNALKADPQATSELQKL
VSPHHPMSSPGYFKDIQIRIQKFVDSGQLGIFKNGYWSNPAYKLSPEADL
MAVTHYLEALDFQKEIVKIHAIFGGKNPHPNYMVGGVPCAINIDGDMAAG
APINMERLNFVKSLIEQGRTFNTNVYVPDVIAIAAFYRDWLYGGGLSATN
VMDYGAYPKTPYDKSTDQLPGGAIINGDWGKIHPVDPRDPEQVQEFVTHS
WYKYPDETKGLHPWDGITEPNYELGSKTKGSRTNIIEIDESAKYSWIKSP
RWRGHAVEVGPLARYILAYAQGVEYVKTQVHTSLNRFNAVCRLLDPNHKD
ITDLKAFLGSTIGRTLARALESEYCGDMMLDDFNQLISNIKNGDSSTANT
DKWDPSSWPEHAKGVGTVAAPRGALAHWIVIEKGKIKNYQCVVPTTWNGS
PRDPKGNIGAFEASLMGTPMERPDEPVEVLRTLHSFDPCLACSTH

3D structure

• PDB: 3ayx Structural basis for a [4Fe-3S] cluster in the oxygen-tolerant membrane-bound [NiFe]-hydrogenase
• Chain: C
• Resolution: 1.18 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E28 C76 C79 H83 R523 T546 C590 C593
• Catalytic site (residue number reindexed from 1): E27 C75 C78 H82 R522 T545 C589 C592
• Enzyme Commision number: 1.12.5.1: hydrogen:quinone oxidoreductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	C	E57 C542 H596	E56 C541 H595
BS02	FE2	C	C79 C590 C593	C78 C589 C592
BS03	O	C	R523 C590	R522 C589
BS04	O	C	C76 C590 C593	C75 C589 C592
BS05	O	C	E28 C590 A592	E27 C589 A591
BS06	O	C	C76 C79	C75 C78
BS07	O	C	C76 C590 C593	C75 C589 C592

Gene Ontology

Molecular Function

• GO:0008901 ferredoxin hydrogenase activity
• GO:0016151 nickel cation binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Cellular Component

• GO:0005886 plasma membrane

External links

• PDB: RCSB:3ayx, PDBe:3ayx, PDBj:3ayx
• PDBsum: 3ayx
• PubMed: 22002607
• UniProt: F2Z6J6