Structure of PDB 3ay6 Chain C

Receptor sequence

>3ay6C (length=262) Species: 1404 (Priestia megaterium)

HMYTDLKDKVVVITGGSTGLGRAMAVRFGQEEAKVVINYYNNEEEALDAK
KEVEEAGGQAIIVQGDVTKEEDVVNLVQTAIKEFGTLDVMINNAGVENPV
PSHELSLDNWNKVIDTNLTGAFLGSREAIKYFVENDIKGNVINMSSVHEM
IPWPLFVHYAASKGGMKLMTETLALEYAPKGIRVNNIGPGAMNTPINAEK
FADPVQRADVESMIPMGYIGKPEEVAAVAAFLASSQASYVTGITLFADGG
MTKYPSFQFGRG

3D structure

• PDB: 3ay6 Structure-guided mutagenesis for the improvement of substrate specificity of Bacillus megaterium glucose 1-dehydrogenase IV
• Chain: C
• Resolution: 2.1 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G18 S145 Y158 K162
• Catalytic site (residue number reindexed from 1): G19 S146 Y159 K163
• Enzyme Commision number: 1.1.1.47: glucose 1-dehydrogenase [NAD(P)(+)].

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAI	C	G14 T17 G18 L19 Y39 G64 D65 V66 N92 A93 G94 M143 S145 Y158 K162 P188 G189 M191 T193 P194 I195 N196	G15 T18 G19 L20 Y40 G65 D66 V67 N93 A94 G95 M144 S146 Y159 K163 P189 G190 M192 T194 P195 I196 N197
BS02	BGC	C	E96 S145 H147 W152 Y158 N196 K199	E97 S146 H148 W153 Y159 N197 K200

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0042802 identical protein binding
• GO:0047934 glucose 1-dehydrogenase (NAD+) activity
• GO:0047935 glucose 1-dehydrogenase (NADP+) activity
• GO:0047936 glucose 1-dehydrogenase [NAD(P)+] activity

External links

• PDB: RCSB:3ay6, PDBe:3ay6, PDBj:3ay6
• PDBsum: 3ay6
• PubMed: 22804868
• UniProt: P39485|DHG4_PRIMG Glucose 1-dehydrogenase 4 (Gene Name=gdhIV)