Structure of PDB 2ziz Chain C

Receptor sequence

>2zizC (length=485) Species: 83332 (Mycobacterium tuberculosis H37Rv) [Search protein sequence]

LTPDVRNGIDFKIADLSLADFGRKELRIAEHEMPGLMSLRREYAEVQPLK
GARISGSLHMTVQTAVLIETLTALGAEVRWASCNIFSTQDHAAAAVVVGP
HGTPDEPKGVPVFAWKGETLEEYWWAAEQMLTWPDPDKPANMILDDGGDA
TMLVLRGMQYEKAGVVPPAEEDDPAEWKVFLNLLRTRFETDKDKWTKIAE
SVKGVTEETTTGVLRLYQFAAAGDLAFPAINVNDSVTKSKFDNKYGTRHS
LIDGINRGTDALIGGKKVLICGYGDVGKGCAEAMKGQGARVSVTEIDPIN
ALQAMMEGFDVVTVEEAIGDADIVVTATGNKDIIMLEHIKAMKDHAILGN
IGHFDNEIDMAGLERSGATRVNVKPQVDLWTFGDTGRSIIVLSEGRLLNL
GNATGHPSFVMSNSFANQTIAQIELWTKNDEYDNEVYRLPKHLDEKVARI
HVEALGGHLTKLTKEQAEYLGVDVEGPYKPDHYRY

3D structure

PDB

2ziz Crystal structures of Mycobacterium tuberculosis S-adenosyl-L-homocysteine hydrolase in ternary complex with substrate and inhibitors.

Chain

Resolution

2.2 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	H69 S92 S97 D156 E218 N243 K248 D252 N253 T257 H363 H416 S424 Q428
Catalytic site (residue number reindexed from 1)	H59 S82 S87 D146 E208 N233 K238 D242 N243 T247 H353 H406 S414 Q418
Enzyme Commision number	3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	C	T219 T220 T221 N253 G284 V286 E305 I306 N310 T338 G339 N340 I343 I361 G362 H363 N409 H416	T209 T210 T211 N243 G274 V276 E295 I296 N300 T328 G329 N330 I333 I351 G352 H353 N399 H406
BS02	AD3	C	L68 H69 T71 D156 E218 T219 K248 D252 H363 L410 G415 H416 M421 F425	L58 H59 T61 D146 E208 T209 K238 D242 H353 L400 G405 H406 M411 F415	MOAD: ic50=20uM
BS03	NAD	C	K489 Y493	K479 Y483

Gene Ontology

	Molecular Function
GO:0004013	adenosylhomocysteinase activity
GO:0005515	protein binding
GO:0016787	hydrolase activity
GO:0035375	zymogen binding
GO:0070403	NAD+ binding

	Biological Process
GO:0006730	one-carbon metabolic process
GO:0009087	methionine catabolic process
GO:0033353	S-adenosylmethionine cycle
GO:0035635	entry of bacterium into host cell
GO:0044650	adhesion of symbiont to host cell
GO:0046085	adenosine metabolic process
GO:0071269	L-homocysteine biosynthetic process

	Cellular Component
GO:0005576	extracellular region
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0009274	peptidoglycan-based cell wall

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:2ziz, PDBe:2ziz, PDBj:2ziz
PDBsum	2ziz
PubMed	18815415
UniProt	P9WGV3\|SAHH_MYCTU Adenosylhomocysteinase (Gene Name=ahcY)

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