Structure of PDB 2y0p Chain C

Receptor sequence

>2y0pC (length=856) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

EDKNARVIELIAAYRNRGHLMADIDPLRLDNTRFNSHGLTLWDLDREFKV
DGFAGVQRKKLRDILSVLRDAYCRHVGVEYTHILEPEQQRWIQERVETKH
DKPTVAEQKYILSKLNAAEAFETFLQTKYVGQKRFSLEGAETVIPMMDAV
IDQCAEHGLDEVVIAMPHRGRLNVLANIVGKPYSQIFSEFEGNLNPSQAH
GSGDVKYHLGATGTYIQMFGDNDIEVSLTANPSHLEAVDPVLEGLVRAKQ
DLLDTGEEGSDNRFSVVPLMLHGDAAFAGQGVVAETLNLALLRGYRTGGT
IHIVVNNQIGFTTAPTDSRSSEYCTDVAKMIGAPIFHVNGDDPEACAWVA
RLAVDFRQAFKKDVVIDMLCYRRRGHNEGDDPSMTQPYMYDVIDTKRGSR
KAYTEALIGRGDISMKEAEDALRDYQGQLERVFNEVRELEKHEIEPSESV
EADQQIPSKLATAVDKAMLQRIGDAHLALPEGFTVHPRVRPVLEKRREMA
YEGRIDWAFAELLALGSLIAEGKLVRLSGQDTQRGTFTQRHAVIVDRKTG
EEFTPLQLLATNPDGTPTGGKFLVYNSALSEFAAVGFEYGYSVGNPDAMV
LWEAQFGDFVNGAQSIIDEFISSGEAKWGQLSDVVLLLPHGHEGQGPDHT
SGRIERFLQLWAEGSMTIAMPSTPANYFHLLRRHGKDGIQRPLIVFTPKS
MLRNKAAVSDIRDFTESKFRSVLEEPMYTDGEGDRNKVTRLLLTSGKIYY
ELAARKAKENREDVAIVRIEQLAPLPRRRLAETLDRYPNVKEKFWVQEEP
ANQGAWPSFGLTLPEILPDHFTGLKRISRRAMSAPSSGSSKVHAVEQQEI
LDTAFG

3D structure

• PDB: 2y0p Functional Plasticity and Allosteric Regulation of Alpha-Ketoglutarate Decarboxylase in Central Mycobacterial Metabolism.
• Chain: C
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 1.2.4.2: oxoglutarate dehydrogenase (succinyl-transferring).
  2.2.1.5: 2-hydroxy-3-oxoadipate synthase.
  2.3.1.61: dihydrolipoyllysine-residue succinyltransferase.
  4.1.1.71: 2-oxoglutarate decarboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TD7	C	F506 H539 R540 Y578 H579 S604 L606 G644 D645 A646 A647 N678 I680 H747	F135 H168 R169 Y207 H208 S233 L235 G273 D274 A275 A276 N307 I309 H376
BS02	MG	C	D645 N678 I680	D274 N307 I309
BS03	ACO	C	E822 I827 K830 G1035 M1037 T1038 R1054 S1092 Q1142 P1145 L1146 P1147 R1149 R1150	E451 I456 K459 G664 M666 T667 R683 S721 Q771 P774 L775 P776 R778 R779
BS04	TD7	C	Q901 L950 E952 Q976 F980 H1020	Q530 L579 E581 Q605 F609 H649

Gene Ontology

Molecular Function

• GO:0016624 oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
• GO:0030976 thiamine pyrophosphate binding

External links

• PDB: RCSB:2y0p, PDBe:2y0p, PDBj:2y0p
• PDBsum: 2y0p
• PubMed: 21867916
• UniProt: A0R2B1|KGD_MYCS2 Multifunctional 2-oxoglutarate metabolism enzyme (Gene Name=kgd)