Structure of PDB 2wtx Chain C

Receptor sequence
>2wtxC (length=440) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
SRLVVVSNRIAPGLAVGILGALKAAGGLWFGWSGETGNQPLKKVKKGNIT
WASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPAWDGYLRVNAL
LADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPTPE
IFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSH
TAWGKAFRTEVYPIGIEPKEIAKQAAGPAQLKAELKNVQNIFSVERLDYS
KGLPERFLAYEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENE
AGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLV
AKEYVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDEVAAALDRAL
TMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPR
3D structure
PDB2wtx Mechanistic Insight Into Enzymatic Glycosyl Transfer with Retention of Configuration Through Analysis of Glycomimetic Inhibitors.
ChainC
Resolution2.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H154 D361
Catalytic site (residue number reindexed from 1) H143 D345
Enzyme Commision number 2.4.1.15: alpha,alpha-trehalose-phosphate synthase (UDP-forming).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 UDP C V260 R262 K267 H338 F339 L344 N364 L365 V366 E369 V244 R246 K251 H322 F323 L328 N348 L349 V350 E353
BS02 VDO C D130 H154 I225 R262 R300 D361 G362 M363 N364 D119 H143 I214 R246 R284 D345 G346 M347 N348 MOAD: Ki=1.3mM
PDBbind-CN: -logKd/Ki=2.89,Ki=1.3mM
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0003825 alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity
GO:0016757 glycosyltransferase activity
GO:0016758 hexosyltransferase activity
Biological Process
GO:0005992 trehalose biosynthetic process
GO:0006950 response to stress
GO:0006970 response to osmotic stress
GO:0006974 DNA damage response
GO:0070415 trehalose metabolism in response to cold stress

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Molecular Function
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Biological Process
External links
PDB RCSB:2wtx, PDBe:2wtx, PDBj:2wtx
PDBsum2wtx
PubMed20077550
UniProtP31677|OTSA_ECOLI Trehalose-6-phosphate synthase (Gene Name=otsA)

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