Structure of PDB 2rjp Chain C

Receptor sequence

>2rjpC (length=290) Species: 9606 (Homo sapiens)

SLSRFVETLVVADDKMAAFHGAGLKRYLLTVMAAAAKAFKHPSIRNPVSL
VVTRLVILEGPQVGPSAAQTLRSFCAWQRGLNTPEDSDPDHFDTAILFTR
QDLCGVSTCDTLGMADVGTVCDPARSCAIVEDDGLQSAFTAAHQLGHVFN
MLHDNSKPCISLNGPLSTSRHVMAPVMAHVDPEEPWSPCSARFITDFLDN
GYGHCLLDKPEAPLHLPVTFPGKDYDADRQCQLTFGPDSRHCPQLPPPCA
ALWCSGHLNGHAMCQTKHSPWADGTPCGPAQACMGGRCLH

3D structure

• PDB: 2rjp Crystal structures of the two major aggrecan degrading enzymes, ADAMTS4 and ADAMTS5.
• Chain: C
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 3.4.24.82: ADAMTS-4 endopeptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	C	H361 H365 H371	H143 H147 H153
BS02	CA	C	D320 L321 C327 T329 E349	D102 L103 C109 T111 E131
BS03	CA	C	E221 D304 D311 C423 D426	E7 D86 D93 C205 D208
BS04	CA	C	E221 D304 D426	E7 D86 D208
BS05	886	C	T329 L330 G331 M332 F357 H361 Q362 H365 H371 V390 A392 P393 V394 M395 V398	T111 L112 G113 M114 F139 H143 Q144 H147 H153 V172 A174 P175 V176 M177 V180	BindingDB: IC50=100nM

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0008237 metallopeptidase activity

Biological Process

• GO:0006508 proteolysis

External links

• PDB: RCSB:2rjp, PDBe:2rjp, PDBj:2rjp
• PDBsum: 2rjp
• PubMed: 18042673
• UniProt: O75173|ATS4_HUMAN A disintegrin and metalloproteinase with thrombospondin motifs 4 (Gene Name=ADAMTS4)