Structure of PDB 2qfp Chain C

Receptor sequence

>2qfpC (length=424) Species: 3885 (Phaseolus vulgaris)

RDMPLDSDVFRVPPGYNAPQQVHITQGDLVGRAMIISWVTMDEPGSSAVR
YWSEKNGRKRIAKGKMSTYRFFNYSSGFIHHTTIRKLKYNTKYYYEVGLR
NTTRRFSFITPPQTGLDVPYTFGLIGDLGQSFDSNTTLSHYELSPKKGQT
VLFVGDLSYADRYPNHDNVRWDTWGRFTERSVAYQPWIWTAGNHEIEFAP
EINETEPFKPFSYRYHVPYEASQSTSPFWYSIKRASAHIIVLSSYSAYGR
GTPQYTWLKKELRKVKRSETPWLIVLMHSPLYNSYNHHFMEGEAMRTKFE
AWFVKYKVDVVFAGHVHAYERSERVSNIAYKITNGLCTPVKDQSAPVYIT
IGDAGNYGVIDSNMIQPQPEYSAFREASFGHGMFDIKNRTHAHFSWNRNQ
DGVAVEADSVWFFNRHWYPVDDST

3D structure

• PDB: 2qfp Crystal structures of a purple acid phosphatase, representing different steps of this enzyme's catalytic cycle.
• Chain: C
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D135 D164 Y167 N201 H202 H286 H295 H296 H323 H325
• Catalytic site (residue number reindexed from 1): D127 D156 Y159 N193 H194 H278 H287 H288 H315 H317
• Enzyme Commision number: 3.1.3.2: acid phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FE	C	D135 D164 Y167 H325	D127 D156 Y159 H317
BS02	ZN	C	D164 N201 H286 H323	D156 N193 H278 H315
BS03	SO4	C	H202 H325 Y365	H194 H317 Y357
BS04	F	C	N201 H323	N193 H315

Gene Ontology

Molecular Function

• GO:0003993 acid phosphatase activity
• GO:0008199 ferric iron binding
• GO:0008270 zinc ion binding
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:2qfp, PDBe:2qfp, PDBj:2qfp
• PDBsum: 2qfp
• PubMed: 18234116
• UniProt: P80366|PPAF_PHAVU Fe(3+)-Zn(2+) purple acid phosphatase