Structure of PDB 2q1l Chain C

Receptor sequence

>2q1lC (length=416) Species: 9606 (Homo sapiens)

PNEECLQILGNAEGAKFLSDAEIIQLVNAKHIPAYKLETLIETHERGVSI
RRQLLSKKLSEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPL
CLDEKEFQVPMATTEGCLVASTNRGCRAIGLGGGASSRVLADGMTRGPVV
RLPRACDSAEVKAWLETSEGFAVIKEAFDSTSRFARLQKLHTSIAGRNLY
IRFQSRSGDAMGMNMISKGTEKALSKLHEYFPEMQILAVSGNYCTDKKPA
AINWIEGRGKSVVCEAVIPAKVVREVLKTTTEAMIEVNINKNLVGSAMAG
SIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYIS
CTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCG
TVMAGELSLMAALAAG

3D structure

• PDB: 2q1l Design and synthesis of hepatoselective, pyrrole-based HMG-CoA reductase inhibitors.
• Chain: C
• Resolution: 2.05 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E559 K691 D767
• Catalytic site (residue number reindexed from 1): E115 K247 D323
• Enzyme Commision number: 1.1.1.34: hydroxymethylglutaryl-CoA reductase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	882	C	R590 V683 S684 D690 K692	R146 V239 S240 D246 K248	MOAD: ic50=12.4nM BindingDB: IC50=1.8nM
BS02	882	C	E559 C561 L562 S565 R568 K735 A751 L853 A856 L857	E115 C117 L118 S121 R124 K291 A307 L409 A412 L413	MOAD: ic50=12.4nM BindingDB: IC50=1.8nM

Gene Ontology

Molecular Function

• GO:0004420 hydroxymethylglutaryl-CoA reductase (NADPH) activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0050661 NADP binding

Biological Process

• GO:0008299 isoprenoid biosynthetic process
• GO:0015936 coenzyme A metabolic process

Cellular Component

• GO:0005789 endoplasmic reticulum membrane

External links

• PDB: RCSB:2q1l, PDBe:2q1l, PDBj:2q1l
• PDBsum: 2q1l
• PubMed: 17574412
• UniProt: P04035|HMDH_HUMAN 3-hydroxy-3-methylglutaryl-coenzyme A reductase (Gene Name=HMGCR)