Structure of PDB 1wcq Chain C

Receptor sequence
>1wcqC (length=599) Species: 1881 (Micromonospora viridifaciens) [Search protein sequence]
PLYTEQDLAVNGREGFPNYRIPALTVTPDGDLLASYDGRPTGIDAPGPNS
ILQRRSTDGGRTWGEQQVVSAGQTTAPIKGFSDPSYLVDRETGTIFNFHV
YSQRQGFAGSRPGTDPADPNVLHANVATSTDGGLTWSHRTITADITPDPG
WRSRFAASGEGIQLRYGPHAGRLIQQYTIINAAGAFQAVSVYSDDHGRTW
RAGEAVGVGMDENKTVELSDGRVLLNSRDSARSGYRKVAVSTDGGHSYGP
VTIDRDLPDPTNNASIIRAFPDAPAGSARAKVLLFSNAASQTSRSQGTIR
MSCDDGQTWPVSKVFQPGSMSFSTLTALPDGTYGLLYEPGTGIRYANFNL
AWLGGICAPFTIPDVALEPGQQVTVPVAVTNQSGIAVPKPSLQLDASPDW
QVQGSVEPLMPGRQAKGQVTITVPAGTTPGRYRVGATLRTSAGNASTTFT
VTVGLLDQARMSIADVDSEETAREDGRASNVIDGNPSTFWHTEWSRADAP
GYPHRISLDLGGTHTISGLQYTRRQNSANEQVADYEIYTSLNGTTWDGPV
ASGRFTTSLAPQRAVFPARDARYIRLVALSEQTGHKYAAVAELEVEGQR
3D structure
PDB1wcq Two Nucleophilic Mutants of the Micromonospora Viridifaciens Sialidase Operate with Retention of Configuration by Two Different Mechanisms.
ChainC
Resolution2.1 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D92 E260 F370
Catalytic site (residue number reindexed from 1) D44 E212 F322
Enzyme Commision number 3.2.1.18: exo-alpha-sialidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 DAN C R68 I69 D131 F155 F203 D259 R276 R342 F370 R20 I21 D83 F107 F155 D211 R228 R294 F322 MOAD: Ki=0.55uM
Gene Ontology
Molecular Function
GO:0004308 exo-alpha-sialidase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0006689 ganglioside catabolic process
GO:0009313 oligosaccharide catabolic process
Cellular Component
GO:0005576 extracellular region
GO:0005737 cytoplasm
GO:0016020 membrane
GO:0043231 intracellular membrane-bounded organelle

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1wcq, PDBe:1wcq, PDBj:1wcq
PDBsum1wcq
PubMed16206228
UniProtQ02834|NANH_MICVI Sialidase (Gene Name=nedA)

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