Structure of PDB 1w21 Chain C

Receptor sequence

>1w21C (length=389) Species: 11320 (Influenza A virus)

RTFLNLTKPLCEVNSWHILSKDNAIRIGEDAHILVTREPYLSCDPQGCRM
FALSQGTTLRGRHANGTIHDRSPFRALISWEMGQAPSPYNTRVECIGWSS
TSCHDGMSRMSICMSGPNNNASAVVWYGGRPITEIPSWAGNILRTQESEC
VCHKGVCPVVMTDGPANNRAATKIIYFKEGKIQKIEELAGNAQHIEECSC
YGAGGVIKCICRDNWKGANRPVITIDPEMMTHTSKYLCSKVLTDTSRPND
PTNGNCDAPITGGSPDPGVKGFAFLDGENSWLGRTISKDSRSGYEMLKVP
NAETDIQSGPISNQVIVNNQNWSGYSGAFIDYWANKECFNPCFYVELIRG
RPKESSVLWTSNSIVALCGSKKRLGSWSWHDGAEIIYFE

3D structure

• PDB: 1w21 The Crystal Structure of Type a Influenza Virus Neuraminidase of the N6 Subtype Reveals the Existence of Two Separate Neu5Ac Binding Sites
• Chain: C
• Resolution: 2.08 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R2299 Y2412
• Catalytic site (residue number reindexed from 1): R212 Y325
• Enzyme Commision number: 3.2.1.18: exo-alpha-sialidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BMA	C	I2398 S2399 N2400	I311 S312 N313
BS02	MAN	C	L2384 P2397 N2400	L297 P310 N313
BS03	SIA	C	E2125 D2157 A2253 E2283 E2284 R2299 R2378 Y2412	E38 D70 A166 E196 E197 R212 R291 Y325
BS04	SIA	C	S2374 S2377 S2379 N2406 W2409	S287 S290 S292 N319 W322
BS05	MAN	C	E2382 N2400	E295 N313
BS06	CA	C	D2300 G2304 D2331 P2354	D213 G217 D244 P267

Gene Ontology

Molecular Function

• GO:0004308 exo-alpha-sialidase activity

Biological Process

• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0016020 membrane
• GO:0033644 host cell membrane
• GO:0055036 virion membrane

External links

• PDB: RCSB:1w21, PDBe:1w21, PDBj:1w21
• PDBsum: 1w21
• UniProt: Q6XV27|NRAM_I56A2 Neuraminidase (Gene Name=NA)