Structure of PDB 1umb Chain C

Receptor sequence
>1umbC (length=361) Species: 274 (Thermus thermophilus) [Search protein sequence]
HRFETFTEEPIRLIGEEGEWLGDFPLDLEGEKLRRLYRDMLAARMLDERY
TILIRTGKTSFIAPAAGHEAAQVAIAHAIRPGFDWVFPYYRDHGLALALG
IPLKELLGQMLATKADPNKGRQMPEHPGSKALNFFTVASPIASHVPPAAG
AAISMKLLRTGQVAVCTFGDGATSEGDWYAGINFAAVQGAPAVFIAENNF
YAISVDYRHQTHSPTIADKAHAFGIPGYLVDGMDVLASYYVVKEAVERAR
RGEGPSLVELRVYRYGPHSSADDDSRYRPKEEVAFWRKKDPIPRFRRFLE
ARGLWNEEWEEDVREEIRAELERGLKEAEEAGPVPPEWMFEDVFAEKPWH
LLRQEALLKEE
3D structure
PDB1umb Ligand-induced Conformational Changes and a Reaction Intermediate in Branched-chain 2-Oxo Acid Dehydrogenase (E1) from Thermus thermophilus HB8, as Revealed by X-ray Crystallography
ChainC
Resolution2.1 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) I57 S144 R269 H273 S274 Y282
Catalytic site (residue number reindexed from 1) I52 S139 R264 H268 S269 Y277
Enzyme Commision number 1.2.4.4: 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG C D175 N204 Y206 D170 N199 Y201
BS02 TPP C Y94 Y95 R96 I146 G174 D175 G176 N204 Y206 A207 I208 H273 Y89 Y90 R91 I141 G169 D170 G171 N199 Y201 A202 I203 H268
Gene Ontology
Molecular Function
GO:0003863 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity
GO:0005515 protein binding
GO:0016491 oxidoreductase activity
GO:0016624 oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
GO:0046872 metal ion binding
Biological Process
GO:0009083 branched-chain amino acid catabolic process

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Molecular Function
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Biological Process
External links
PDB RCSB:1umb, PDBe:1umb, PDBj:1umb
PDBsum1umb
PubMed15033367
UniProtQ5SLR4|ODBA_THET8 2-oxoisovalerate dehydrogenase subunit alpha (Gene Name=TTHA0229)

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