Structure of PDB 1rr8 Chain C

Receptor sequence

>1rr8C (length=494) Species: 9606 (Homo sapiens)

AAWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLS
PKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKC
DFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERI
ANFKIEPPGLSRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHK
WKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRL
KKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETAD
TVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKN
LQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQ
QLKELTAPDENIPAKILSYNRANRAVAILCNHQQAPREENKQIALGTSKL
NYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF

3D structure

• PDB: 1rr8 Mechanisms of camptothecin resistance by human topoisomerase I mutations
• Chain: C
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.1: DNA topoisomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	T313 V410 T411 W412 I424 Y426 K443 K532 A635 A715 G717 T718 N722 Y723	T113 V210 T211 W212 I224 Y226 K243 K332 A435 A444 G446 T447 N451 Y452
BS02	dna	C	R362 G363 R364 H367 R488 A489 N491 K493 T501 K532 D533 T585 A586 K587	R162 G163 R164 H167 R288 A289 N291 K293 T301 K332 D333 T385 A386 K387
BS03	TTG	C	R364 K532 D533 T718 N722	R164 K332 D333 T447 N451
BS04	TTC	C	E356 R364 K532 D533 T718	E156 R164 K332 D333 T447	BindingDB: EC50=54nM,IC50=5250nM,Kd=26.5nM

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity

Biological Process

• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:1rr8, PDBe:1rr8, PDBj:1rr8
• PDBsum: 1rr8
• PubMed: 15165849
• UniProt: P11387|TOP1_HUMAN DNA topoisomerase 1 (Gene Name=TOP1)