Structure of PDB 1pj4 Chain C

Receptor sequence

>1pj4C (length=552) Species: 9606 (Homo sapiens)

KEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRN
LKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACS
QYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGD
LGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGL
YQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYRE
KYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIAN
LIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPE
SIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSN
PTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIF
PGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQE
VSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYE
WP

3D structure

• PDB: 1pj4 Crystal structures of substrate complexes of malic enzyme and insights into the catalytic mechanism.
• Chain: C
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y2112 R2165 K2183 E2255 D2256 D2278 D2279 N2421
• Catalytic site (residue number reindexed from 1): Y91 R144 K162 E234 D235 D257 D258 N400
• Enzyme Commision number: 1.1.1.38: malate dehydrogenase (oxaloacetate-decarboxylating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MN	C	E2255 D2256 D2279	E234 D235 D258
BS02	ATP	C	R2165 N2259 A2312 G2313 E2314 A2315 D2345 K2346 V2392 A2393	R144 N238 A291 G292 E293 A294 D324 K325 V371 A372
BS03	ATP	C	I2193 R2194 R2197 I2479 L2480 R2542 Y2552 R2556	I172 R173 R176 I458 L459 R521 Y531 R535
BS04	FUM	C	Q2064 R2067 R2091 L2095	Q43 R46 R70 L74

Gene Ontology

Molecular Function

• GO:0004470 malic enzyme activity
• GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
• GO:0004473 malate dehydrogenase (decarboxylating) (NADP+) activity
• GO:0008948 oxaloacetate decarboxylase activity
• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0046872 metal ion binding
• GO:0051287 NAD binding

Biological Process

• GO:0006090 pyruvate metabolic process
• GO:0006108 malate metabolic process
• GO:1902031 regulation of NADP metabolic process

Cellular Component

• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:1pj4, PDBe:1pj4, PDBj:1pj4
• PDBsum: 1pj4
• PubMed: 12962632
• UniProt: P23368|MAOM_HUMAN NAD-dependent malic enzyme, mitochondrial (Gene Name=ME2)