Structure of PDB 1pj2 Chain C

Receptor sequence

>1pj2C (length=553) Species: 9606 (Homo sapiens) [Search protein sequence]

IKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHR
NLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLAC
SQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLG
DLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMG
LYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYR
EKYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIA
NLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAP
ESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALS
NPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYI
FPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQ
EVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVY
EWP

3D structure

PDB

1pj2 Crystal structures of substrate complexes of malic enzyme and insights into the catalytic mechanism.

Chain

Resolution

2.3 Å

3D
structure

Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]

Enzymatic activity

Catalytic site (original residue number in PDB)	Y2112 R2165 K2183 E2255 D2256 D2278 D2279 N2421
Catalytic site (residue number reindexed from 1)	Y92 R145 K163 E235 D236 D258 D259 N401
Enzyme Commision number	1.1.1.38: malate dehydrogenase (oxaloacetate-decarboxylating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	LMR	C	Y2112 T2113 R2165 L2167 K2183 D2279 N2421 N2466 N2467	Y92 T93 R145 L147 K163 D259 N401 N446 N447
BS02	MN	C	E2255 D2256 D2279	E235 D236 D259
BS03	NAI	C	R2165 L2167 G2168 N2259 T2283 G2311 A2312 G2313 E2314 D2345 K2346 V2392 A2393 G2394 L2398 L2419 N2421 G2465 N2466 N2467	R145 L147 G148 N239 T263 G291 A292 G293 E294 D325 K326 V372 A373 G374 L378 L399 N401 G445 N446 N447
BS04	NAI	C	H2154 K2156 I2193 R2194 L2480 R2542 Y2552 R2556	H134 K136 I173 R174 L460 R522 Y532 R536
BS05	FUM	C	R2067 R2091	R47 R71

Gene Ontology

	Molecular Function
GO:0004470	malic enzyme activity
GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
GO:0004473	malate dehydrogenase (decarboxylating) (NADP+) activity
GO:0008948	oxaloacetate decarboxylase activity
GO:0009055	electron transfer activity
GO:0016491	oxidoreductase activity
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0046872	metal ion binding
GO:0051287	NAD binding

	Biological Process
GO:0006090	pyruvate metabolic process
GO:0006108	malate metabolic process
GO:1902031	regulation of NADP metabolic process

	Cellular Component
GO:0005739	mitochondrion
GO:0005759	mitochondrial matrix
GO:0043231	intracellular membrane-bounded organelle

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:1pj2, PDBe:1pj2, PDBj:1pj2
PDBsum	1pj2
PubMed	12962632
UniProt	P23368\|MAOM_HUMAN NAD-dependent malic enzyme, mitochondrial (Gene Name=ME2)

[Back to BioLiP]