Structure of PDB 1gy8 Chain C

Receptor sequence

>1gy8C (length=370) Species: 5691 (Trypanosoma brucei)

SHMRVLVCGGAGYIGSHFVRALLRDTNHSVVIVDSLVGTHGKSDHVETRE
NVARKLQQSDGPKPPWADRYAALEVGDVRNEDFLNGVFTRHGPIDAVVHM
CAFLAVGESVRDPLKYYDNNVVGILRLLQAMLLHKCDKIIFSSSAAIFGN
PTMNAEPIDINAKKSPESPYGESKLIAERMIRDCAEAYGIKGICLRYFNA
CGAHEDGDIGEHYQGSTHLIPIILGRVMSDIAPDASTDKRMPIFGTDYPT
PDGTCVRDYVHVCDLASAHILALDYVEKLGPNDKSKYFSVFNLGTSRGYS
VREVIEVARKTTGHPIPVRECGRREGDPAYLVAASDKAREVLGWKPKYDT
LEAIMETSWKFQRTHPNGYA

3D structure

• PDB: 1gy8 High-Resolution Crystal Structure of Trypanosoma Brucei Udp-Galactose 4'-Epimerase: A Potential Target for Structure-Based Development of Novel Trypanocides
• Chain: C
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S142 A143 A144 Y173 K177
• Catalytic site (residue number reindexed from 1): S144 A145 A146 Y170 K174
• Enzyme Commision number: 5.1.3.2: UDP-glucose 4-epimerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	C	G7 G10 Y11 I12 D32 S33 L34 V35 G36 D75 V76 M98 C99 A100 L102 N117 S140 S141 S142 Y173 K177 Y200 A203	G9 G12 Y13 I14 D34 S35 L36 V37 G38 D77 V78 M100 C101 A102 L104 N119 S142 S143 S144 Y170 K174 Y197 A200
BS02	UDP	C	N202 H221 L222 I225 M252 P253 I254 F255 C266 R268 R335 D338	N199 H218 L219 I222 M241 P242 I243 F244 C255 R257 R324 D327

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003978 UDP-glucose 4-epimerase activity
• GO:0016853 isomerase activity

Biological Process

• GO:0006012 galactose metabolic process
• GO:0033499 galactose catabolic process via UDP-galactose

Cellular Component

• GO:0005829 cytosol
• GO:0020015 glycosome

External links

• PDB: RCSB:1gy8, PDBe:1gy8, PDBj:1gy8
• PDBsum: 1gy8
• PubMed: 12615316
• UniProt: Q8T8E9