Structure of PDB 1fsi Chain C

Receptor sequence
>1fsiC (length=174) Species: 3702 (Arabidopsis thaliana) [Search protein sequence]
MEEVKKDVYSVWALPDEESEPRFKKLMEALRSEFTGPRFVPHVTVAVSAY
LTADEAKKMFESACDGLKAYTATVDRVSTGTFFFQCVFLLLQTTPEVMEA
GEHCCSTPYMPHLSLLYAELTEEEKKNAQEKAYTLDSSLDGLSFRLNRLA
LCKTDTEDKTLETWETVAVCNLNP
3D structure
PDB1fsi Structure and mechanism of activity of the cyclic phosphodiesterase of Appr>p, a product of the tRNA splicing reaction.
ChainC
Resolution2.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H42 T44 M117 H119 S121 Y124
Catalytic site (residue number reindexed from 1) H42 T44 M110 H112 S114 Y117
Enzyme Commision number 3.1.4.37: 2',3'-cyclic-nucleotide 3'-phosphodiesterase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SO4 C R31 R38 R31 R38
BS02 SO4 C H42 T44 S121 Y124 H42 T44 S114 Y117
BS03 SO4 C R76 S78 Q136 R76 S78 Q129
BS04 SO4 C K5 D7 K160 E172 K5 D7 K153 E165
Gene Ontology
Molecular Function
GO:0004112 cyclic-nucleotide phosphodiesterase activity
GO:0016787 hydrolase activity
Biological Process
GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1fsi, PDBe:1fsi, PDBj:1fsi
PDBsum1fsi
PubMed11080166
UniProtO04147|CPD_ARATH Cyclic phosphodiesterase (Gene Name=At4g18930)

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