Structure of PDB 1ejr Chain C

Receptor sequence

>1ejrC (length=553)

SNISRQAYADMFGPTVGDKVRLADTELWIEVEDDLTTYGEEVKFGGGKVI
RDGMGQGQMLAADCVDLVLTNALIVDHWGIVKADIGVKDGRIFAIGKAGN
PDIQPNVTIPIGAATEVIAAEGKIVTAGGIDTHIHWICPQQAEEALVSGV
TTMVGGGTGPAAGTHATTCTPGPWYISRMLQAADSLPVNIGLLGKGNVSQ
PDALREQVAAGVIGLKIHEAWGATPAAIDCALTVADEMDIQVALHSDTLN
ESGFVEDTLAAIGGRTIHTFHTEGAGGGHAPDIITACAHPNILPSSTNPT
LPYTLNTIDEHLDMLMAFAESRIRRETIAAEDVLHDLGAFSLTSSDSQAM
GRVGEVILRTWQVAHRMKVQRGALAEETGDNDNFRVKRYIAKYTINPALT
HGIAHEVGSIEVGKLADLVVWSPAFFGVKPATVIKGGMIAIAPMGDINAS
IPTPQPVHYRPMFGALGSARHHCRLTFLSQAAAANGVAERLNLRSAIAVV
KGCRTVQKADMVHNSLQPNITVDAQTYEVRVDGELITSEPADVLPMAQRY
FLF

3D structure

• PDB: 1ejr Kinetic and structural characterization of urease active site variants.
• Chain: C
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H1134 H1136 K1217 H1219 A1221 H1246 H1272 R1336 D1360
• Catalytic site (residue number reindexed from 1): H133 H135 K216 H218 A220 H245 H271 R322 D346
• Enzyme Commision number: 3.5.1.5: urease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NI	C	K1217 H1246 H1272	K216 H245 H271
BS02	NI	C	H1134 H1136 K1217 D1360	H133 H135 K216 D346

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0009039 urease activity
• GO:0016151 nickel cation binding
• GO:0016787 hydrolase activity
• GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
• GO:0046872 metal ion binding

Biological Process

• GO:0043419 urea catabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1ejr, PDBe:1ejr, PDBj:1ejr
• PDBsum: 1ejr
• PubMed: 10913264
• UniProt: P18314|URE1_KLEAE Urease subunit alpha (Gene Name=ureC)