Structure of PDB 1eji Chain C

Receptor sequence
>1ejiC (length=478) Species: 10090 (Mus musculus) [Search protein sequence]
MADRDATLWASHEKMLSQPLKDSDAEVYSIIKKESNRQRVGLELIASENF
ASRAVLEALGSSLNNKYSEGYPGQRYYGGTEFIDELEMLCQKRALQAYHL
DPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTD
KKKISATSIFFESMPYKVYPETGYINYDQLEENASLFHPKLIIAGTSCYS
RNLDYARLRKIADDNGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTH
KTLRGCRAGMIFYRKGVRSVDPKTGKETYYELESLINSAVFPGLQGGPHN
HAIAGVAVALKQAMTTEFKIYQLQVLANCRALSDALTELGYKIVTGGSDN
HLILMDLRSKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSGLRLGTP
ALTSRGLLEEDFQKVAHFIHRGIELTLQIQSHMATKATLKEFKEKLAGDE
KIQSAVATLREEVENFASNFSLPGLPDF
3D structure
PDB1eji Structure of a murine cytoplasmic serine hydroxymethyltransferase quinonoid ternary complex: evidence for asymmetric obligate dimers.
ChainC
Resolution2.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Y73 E75 D228 T254 K257 R263
Catalytic site (residue number reindexed from 1) Y67 E69 D222 T248 K251 R257
Enzyme Commision number 2.1.2.1: glycine hydroxymethyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PLG C S53 S119 G120 S121 H148 D228 A230 H231 T254 H256 K257 S47 S113 G114 S115 H142 D222 A224 H225 T248 H250 K251
BS02 PLG C Y73 Y83 G302 G303 Y67 Y77 G296 G297
BS03 THF C E75 Y82 E69 Y76
Gene Ontology
Molecular Function
GO:0000900 mRNA regulatory element binding translation repressor activity
GO:0004372 glycine hydroxymethyltransferase activity
GO:0016740 transferase activity
GO:0030170 pyridoxal phosphate binding
GO:0036094 small molecule binding
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0048027 mRNA 5'-UTR binding
GO:0070905 serine binding
Biological Process
GO:0006231 dTMP biosynthetic process
GO:0006544 glycine metabolic process
GO:0006563 L-serine metabolic process
GO:0006565 L-serine catabolic process
GO:0006730 one-carbon metabolic process
GO:0009113 purine nucleobase biosynthetic process
GO:0017148 negative regulation of translation
GO:0019264 glycine biosynthetic process from serine
GO:0035999 tetrahydrofolate interconversion
GO:0046653 tetrahydrofolate metabolic process
GO:0046655 folic acid metabolic process
GO:0051289 protein homotetramerization
GO:1904482 cellular response to tetrahydrofolate
GO:1990830 cellular response to leukemia inhibitory factor
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1eji, PDBe:1eji, PDBj:1eji
PDBsum1eji
PubMed11063567
UniProtP50431|GLYC_MOUSE Serine hydroxymethyltransferase, cytosolic (Gene Name=Shmt1)

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