Structure of PDB 1do8 Chain C

Receptor sequence

>1do8C (length=553) Species: 9606 (Homo sapiens)

IKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHR
NLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLAC
SQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLG
DLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMG
LYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYR
EKYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIA
NLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAP
ESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALS
NPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYI
FPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQ
EVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVY
EWP

3D structure

• PDB: 1do8 Structure of a closed form of human malic enzyme and implications for catalytic mechanism.
• Chain: C
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y2112 R2165 K2183 E2255 D2256 D2278 D2279 N2421
• Catalytic site (residue number reindexed from 1): Y92 R145 K163 E235 D236 D258 D259 N401
• Enzyme Commision number: 1.1.1.38: malate dehydrogenase (oxaloacetate-decarboxylating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	OXL	C	R2165 L2167 K2183 N2421	R145 L147 K163 N401
BS02	MN	C	E2255 D2256 D2279	E235 D236 D259
BS03	NAD	C	R2165 L2167 G2168 N2259 T2283 G2311 A2312 G2313 E2314 A2315 D2345 K2346 V2392 A2393 A2395 L2419 N2421 N2466 N2467	R145 L147 G148 N239 T263 G291 A292 G293 E294 A295 D325 K326 V372 A373 A375 L399 N401 N446 N447
BS04	NAD	C	H2154 I2193 R2194 R2197 I2479 L2480 R2542 Y2552 R2556	H134 I173 R174 R177 I459 L460 R522 Y532 R536

Gene Ontology

Molecular Function

• GO:0004470 malic enzyme activity
• GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
• GO:0004473 malate dehydrogenase (decarboxylating) (NADP+) activity
• GO:0008948 oxaloacetate decarboxylase activity
• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0046872 metal ion binding
• GO:0051287 NAD binding

Biological Process

• GO:0006090 pyruvate metabolic process
• GO:0006108 malate metabolic process
• GO:1902031 regulation of NADP metabolic process

Cellular Component

• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:1do8, PDBe:1do8, PDBj:1do8
• PDBsum: 1do8
• PubMed: 10700286
• UniProt: P23368|MAOM_HUMAN NAD-dependent malic enzyme, mitochondrial (Gene Name=ME2)