Structure of PDB 1ct9 Chain C

Receptor sequence
>1ct9C (length=495) Species: 562 (Escherichia coli) [Search protein sequence]
ASIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNAILAHERL
SIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCE
VILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYD
EHGQLYVASEMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDA
VKDNVTDKNELRQALEDSVKSHLMSDVPYGVLLSGGLDSSIISAITKKYA
WPQLHSFAVGLPGSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIY
HIETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLYFHKA
PNAKELHEETVRKLLALHMYDCARANKAMSAWGVEARVPFLDKKFLDVAM
RINPQDKMCKMEKHILRECFEAYLPASVAWRQDGVGYSWIDTLKEVAAQQ
VSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPLPSAAECVPG
3D structure
PDB1ct9 Three-dimensional structure of Escherichia coli asparagine synthetase B: a short journey from substrate to product.
ChainC
Resolution2.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) A1 L50 N74 G75 T321 R324 E348 M382
Catalytic site (residue number reindexed from 1) A1 L50 N74 G75 T308 R311 E335 M369
Enzyme Commision number 6.3.5.4: asparagine synthase (glutamine-hydrolyzing).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 AMP C L233 S234 S239 F270 A271 V272 M332 S346 G347 E348 D384 L233 S234 S239 F257 A258 V259 M319 S333 G334 E335 D371
BS02 GLN C A1 R49 L50 I52 V53 N74 G75 E76 D98 C99 A1 R49 L50 I52 V53 N74 G75 E76 D98 C99
Gene Ontology
Molecular Function
GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity
GO:0004071 aspartate-ammonia ligase activity
GO:0005524 ATP binding
GO:0016597 amino acid binding
GO:0016874 ligase activity
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
Biological Process
GO:0006529 asparagine biosynthetic process
GO:0006541 glutamine metabolic process
GO:0008652 amino acid biosynthetic process
GO:0009063 amino acid catabolic process
GO:0070981 L-asparagine biosynthetic process
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1ct9, PDBe:1ct9, PDBj:1ct9
PDBsum1ct9
PubMed10587437
UniProtP22106|ASNB_ECOLI Asparagine synthetase B [glutamine-hydrolyzing] (Gene Name=asnB)

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