Structure of PDB 8ova Chain Bx

Receptor sequence

>8ovaBx (length=235) Species: 5702 (Trypanosoma brucei brucei)

APSPFVARPKDFGIGRDVPYARDLSRFMRWPTFVTMQRKKRVLQRRLKVP
PALNQFTKVLDRSSRNELLKLVKKYAPETRKARRDRLTKVAEEKKKNPTK
APLCVVSGLQEVTRTIEKKTARLVLIANNVDPIELVLWMPTLCRANKIPY
AIVKDKARLGDAIGRKTATCVAFTDVNAEDQAALKNLTRSVNARFLARSD
VIRRQWGGLQLSLRSRAELRKKRARTAGNDAAAKA

3D structure

• PDB: 8ova A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma brucei.
• Chain: Bx
• Resolution: 2.47 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	Bx	R64 T67 R115 R118 G148 Q150 E151 R154 K158 P172 L175 K196 R205 K206 T207 T209	R29 T32 R80 R83 G108 Q110 E111 R114 K118 P132 L135 K156 R165 K166 T167 T169
BS02	rna	Bx	V41 R43 K45 D46 F47 R51 Y55 R57 L59 R61 F62 K74 R76 V77 R80 R81 V94 D96 R97 R229 A233 R234 L236 R238 L253 R254 R260 K261 R263	V6 R8 K10 D11 F12 R16 Y20 R22 L24 R26 F27 K39 R41 V42 R45 R46 V59 D61 R62 R189 A193 R194 L196 R198 L213 R214 R220 K221 R223
BS03	rna	Bx	R61 Q72 R73 R100 K194 D195 R198	R26 Q37 R38 R65 K154 D155 R158

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000470 maturation of LSU-rRNA
• GO:0006412 translation

Cellular Component

• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8ova, PDBe:8ova, PDBj:8ova
• PDBsum: 8ova
• PubMed: 37985661
• UniProt: Q57VM1